Nouioura Ghizlane, Tourabi Meryem, Tahraoui Adel, El-Yagoubi Karima, Maache Souad, Elfatemi Hinde, Lyoussi Badiaa, Derwich El Houssine
Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health and Quality of Life (SNAMOPEQ), Faculty of Sciences Dhar El-Mehraz, Sidi Mohamed Ben Abdellah University, Fez 30 000, Morocco.
Department of Biology and Earth Sciences, Regional Center for Education Careers and Train-ing of Fez-Meknes, Fez 30000, Morocco.
Saudi Pharm J. 2023 Aug;31(8):101701. doi: 10.1016/j.jsps.2023.101701. Epub 2023 Jul 7.
L. is thought to possess a wide range of therapeutic qualities. This plant's safety is critical regarding its potential uses as a medicine. Using the techniques outlined in the OECD recommendations, the present study aimed to assess the acute and subacute toxicity profiles of aqueous extract (FC-Ext) in mice. In the acute study, the FC-Ext was administered to adult male and female Swiss albino mice through oral and intraperitoneal routes at doses of 0-4 g/kg. The general behavioral effects, mortality rates, and latency of mortality were evaluated for a period of 14 days. For the sub-acute dose study, the FC-Ext was administered orally to adult mice at doses of 125, 250, and 500 mg/kg on a daily basis for 28 days. Body weight and selected biochemical and hematological parameters were measured, and histological examinations of the liver, kidney, and spleen were conducted to assess any signs of organ damage at the end of the treatment period. The results of the acute toxicity study demonstrated that the LD values for the oral and intraperitoneal administration of FC-Ext were 3.6 g/kg and 2.3 g/kg, respectively. In the subacute toxicity study of FC-Ext, no significant changes in body weight were observed. However, a substantial increase in the weights of the liver, kidney, and spleen was observed in male mice. The administration of FC-Ext to mice at doses higher than 250 mg/kg resulted in a decrease in white blood cells and platelets in both sexes and a reduction in red blood cells and mean corpuscular hemoglobin concentration in males and hemoglobin in females. No changes in biochemical parameters were observed. Microscopic examination of vital organs such as the liver, kidney, and spleen revealed no significant injuries. Based on the current results, the aqueous extract of has low toxicity. These findings provide important information about the toxicity profile of the traditional medicine plant .
人们认为L.具有广泛的治疗特性。就其作为药物的潜在用途而言，这种植物的安全性至关重要。本研究采用经合组织建议中概述的技术，旨在评估水提取物（FC-Ext）对小鼠的急性和亚急性毒性特征。在急性研究中，将FC-Ext以0 - 4 g/kg的剂量通过口服和腹腔注射途径给予成年雄性和雌性瑞士白化小鼠。在14天的时间内评估一般行为影响、死亡率和死亡潜伏期。对于亚急性剂量研究，将FC-Ext以125、250和500 mg/kg的剂量每日口服给予成年小鼠，持续28天。测量体重以及选定的生化和血液学参数，并在治疗期结束时对肝脏、肾脏和脾脏进行组织学检查，以评估任何器官损伤迹象。急性毒性研究结果表明，FC-Ext口服和腹腔注射的LD值分别为3.6 g/kg和2.3 g/kg。在FC-Ext的亚急性毒性研究中，未观察到体重有显著变化。然而，在雄性小鼠中观察到肝脏、肾脏和脾脏重量大幅增加。以高于250 mg/kg的剂量给小鼠施用FC-Ext导致两性白细胞和血小板减少，雄性红细胞和平均红细胞血红蛋白浓度降低，雌性血红蛋白降低。未观察到生化参数有变化。对肝脏、肾脏和脾脏等重要器官的显微镜检查未发现明显损伤。根据目前的结果，[植物名称]的水提取物毒性较低。这些发现提供了有关这种传统药用植物毒性特征的重要信息。
