Tistechok Stepan, Stierhof Marc, Myronovskyi Maksym, Zapp Josef, Gromyko Oleksandr, Luzhetskyy Andriy
Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, 79005 Lviv, Ukraine.
Department of Pharmaceutical Biotechnology, Saarland University, 66123 Saarbruecken, Germany.
Antibiotics (Basel). 2022 Nov 10;11(11):1587. doi: 10.3390/antibiotics11111587.
Actinomycetes are the most prominent group of microorganisms that produce biologically active compounds. Among them, special attention is focused on bacteria in the genus . Streptomycetes are an important source of biologically active natural compounds that could be considered therapeutic agents. In this study, we described the identification, purification, and structure elucidation of two new naphthoquinone-based meroterpenoids, furaquinocins K and L, from sp. Je 1-369 strain, which was isolated from the rhizosphere soil of (Bieb.). The main difference between furaquinocins K and L and the described furaquinocins was a modification in the polyketide naphthoquinone skeleton. In addition, the structure of furaquinocin L contained an acetylhydrazone fragment, which is quite rare for natural compounds. We also identified a furaquinocin biosynthetic gene cluster in the Je 1-369 strain, which showed similarity (60%) with the furaquinocin B biosynthetic gene cluster from sp. KO-3988. Furaquinocin L showed activity against Gram-positive bacteria without cytotoxic effects.
放线菌是产生生物活性化合物的最主要微生物类群。其中，特别受到关注的是 属的细菌。链霉菌是具有生物活性的天然化合物的重要来源，这些化合物可被视为治疗剂。在本研究中，我们描述了从 （Bieb.）根际土壤中分离出的Je 1-369菌株中两种新的基于萘醌的杂萜类化合物呋喃醌霉素K和L的鉴定、纯化及结构解析。呋喃醌霉素K和L与已报道的呋喃醌霉素的主要区别在于聚酮萘醌骨架的修饰。此外，呋喃醌霉素L的结构包含一个乙酰腙片段，这在天然化合物中相当罕见。我们还在Je 1-369菌株中鉴定出一个呋喃醌霉素生物合成基因簇，它与来自 属KO-3988菌株的呋喃醌霉素B生物合成基因簇具有60%的相似性。呋喃醌霉素L对革兰氏阳性菌具有活性且无细胞毒性作用。
