Vítek Libor, Jirásková Alena, Malíková Ivana, Dostálová Gabriela, Eremiášová Lenka, Danzig Vilém, Linhart Aleš, Haluzík Martin
Institute of Medical Biochemistry and Laboratory Diagnostics, 1st Faculty of Medicine, Charles University and General University Hospital in Prague, Kateřinská 32, 121 08 Prague, Czech Republic.
4th Department of Internal Medicine, 1st Faculty of Medicine, Charles University and General University Hospital in Prague, U. Nemocnice 2, 128 08 Prague, Czech Republic.
Antioxidants (Basel). 2022 Oct 27;11(11):2118. doi: 10.3390/antiox11112118.
Oxidative stress and inflammation contribute significantly to atherogenesis. We and others have demonstrated that mildly elevated serum bilirubin levels protect against coronary and peripheral atherosclerosis, most likely due to the antioxidant and anti-inflammatory activities of bilirubin. The aim of the present study was to assess serum bilirubin and the markers of oxidative stress and inflammation in both healthy subjects and patients with various forms of atherosclerosis. The study was performed in patients with premature myocardial infarction (n = 129), chronic ischemic heart disease (n = 43), peripheral artery disease (PAD, n = 69), and healthy subjects (n = 225). In all subjects, standard serum biochemistry, UGT1A1 genotypes, total antioxidant status (TAS), and concentrations of various pro- and anti-inflammatory chemokines were determined. Compared to controls, all atherosclerotic groups had significantly lower serum bilirubin and TAS, while having much higher serum high-sensitivity C-reactive protein (hsCRP) and most of the analyzed proinflammatory cytokines (p < 0.05 for all comparisons). Surprisingly, the highest inflammation, and the lowest antioxidant status, together with the lowest serum bilirubin, was observed in PAD patients, and not in premature atherosclerosis. In conclusion, elevated serum bilirubin is positively correlated with TAS, and negatively related to inflammatory markers. Compared to healthy subjects, patients with atherosclerosis have a much higher degree of oxidative stress and inflammation.
氧化应激和炎症在动脉粥样硬化的发生发展过程中起着重要作用。我们及其他研究人员已证实，血清胆红素水平轻度升高可预防冠状动脉和外周动脉粥样硬化，这很可能归因于胆红素的抗氧化和抗炎活性。本研究旨在评估健康受试者以及患有各种形式动脉粥样硬化的患者的血清胆红素水平以及氧化应激和炎症标志物。该研究纳入了患有早发性心肌梗死的患者（n = 129）、慢性缺血性心脏病患者（n = 43）、外周动脉疾病（PAD，n = 69）患者以及健康受试者（n = 225）。对所有受试者测定了标准血清生化指标、UGT1A1基因型、总抗氧化状态（TAS）以及各种促炎和抗炎趋化因子的浓度。与对照组相比，所有动脉粥样硬化组的血清胆红素和TAS均显著降低，而血清高敏C反应蛋白（hsCRP）以及大多数分析的促炎细胞因子水平则显著升高（所有比较的p值均 < 0.05）。令人惊讶的是，在PAD患者中观察到炎症程度最高、抗氧化状态最低以及血清胆红素水平最低，而不是在早发性动脉粥样硬化患者中。总之，血清胆红素升高与TAS呈正相关，与炎症标志物呈负相关。与健康受试者相比，动脉粥样硬化患者的氧化应激和炎症程度要高得多。
