Marín-Payá Juan Carlos, Clara-Trujillo Sandra, Cordón Lourdes, Gallego Ferrer Gloria, Sempere Amparo, Gómez Ribelles José Luis
Centre for Biomaterials and Tissue Engineering, CBIT, Universitat Politècnica de València, 46022 Valencia, Spain.
Biomedical Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Valencia, Spain.
Biomedicines. 2022 Nov 3;10(11):2797. doi: 10.3390/biomedicines10112797.
Multiple myeloma is a hematologic neoplasm caused by an uncontrolled clonal proliferation of neoplastic plasma cells (nPCs) in the bone marrow. The development and survival of this disease is tightly related to the bone marrow environment. Proliferation and viability of nPCs depend on their interaction with the stromal cells and the extracellular matrix components, which also influences the appearance of drug resistance. Recapitulating these interactions in an in vitro culture requires 3D environments that incorporate the biomolecules of interest. In this work, we studied the proliferation and viability of three multiple myeloma cell lines in a microgel consisting of biostable microspheres with fibronectin (FN) on their surfaces. We also showed that the interaction of the RPMI8226 cell line with FN induced cell arrest in the G0/G1 cell cycle phase. RPMI8226 cells developed a significant resistance to dexamethasone, which was reduced when they were treated with dexamethasone and bortezomib in combination.
多发性骨髓瘤是一种血液系统肿瘤，由骨髓中肿瘤性浆细胞（nPCs）不受控制的克隆性增殖引起。这种疾病的发展和存活与骨髓环境密切相关。nPCs的增殖和活力取决于它们与基质细胞和细胞外基质成分的相互作用，这也会影响耐药性的出现。在体外培养中重现这些相互作用需要包含感兴趣生物分子的三维环境。在这项工作中，我们研究了三种多发性骨髓瘤细胞系在由表面带有纤连蛋白（FN）的生物稳定微球组成的微凝胶中的增殖和活力。我们还表明，RPMI8226细胞系与FN的相互作用诱导细胞停滞在G0/G1细胞周期阶段。RPMI8226细胞对地塞米松产生了显著耐药性，当它们联合使用地塞米松和硼替佐米治疗时，耐药性降低。
