Corsaro Daniele
CHLAREAS, 54500 Vandoeuvre-lès-Nancy, France.
Microorganisms. 2022 Oct 31;10(11):2162. doi: 10.3390/microorganisms10112162.
is a ubiquitous free-living amoeba capable of being an opportunistic pathogen in humans and animals. A critical step in infection is the adhesion of the amoeba to host cells and tissues, and two major parasite adhesins, mannose-binding protein (MBP) and laminin-binding protein (LBP), are known to recognize the cell surface glycoproteins and those of the extracellular matrix, respectively. In this study, the available genomes of were analysed to recover the sequences of MBP and LBP using previously published genetic data. Genes for both proteins were successfully obtained from strains belonging to various genotypes (T4A, T4D, T4G, T4F, T2, T5, T10, T22, T7 and T18), resulting in a single gene for LBP but identifying two types of MBP, MBP1 and MBP2. Phylogenetic analysis based on deduced amino acid sequences shows that both MBP and LBP have a branching pattern that is consistent with that based on 18S rDNA, indicating that changes in both proteins occurred during diversification of lines. Notably, all MBPs possess a conserved motif, shared with some bacterial C-type lectins, which could be the recognition site for mannose binding.
是一种无处不在的自由生活变形虫，能够成为人类和动物的机会性病原体。感染的关键步骤是变形虫与宿主细胞和组织的粘附，已知两种主要的寄生虫粘附素，即甘露糖结合蛋白（MBP）和层粘连蛋白结合蛋白（LBP），分别识别细胞表面糖蛋白和细胞外基质的糖蛋白。在本研究中，利用先前发表的遗传数据对可用的基因组进行分析，以恢复MBP和LBP的序列。成功从属于各种基因型（T4A、T4D、T4G、T4F、T2、T5、T10、T22、T7和T18）的菌株中获得了这两种蛋白质的基因，结果获得了一个LBP基因，但鉴定出两种类型的MBP，即MBP1和MBP2。基于推导氨基酸序列的系统发育分析表明，MBP和LBP都具有与基于18S rDNA的分支模式一致的分支模式，这表明两种蛋白质的变化发生在品系多样化过程中。值得注意的是，所有的MBP都具有一个与一些细菌C型凝集素共有的保守基序，这可能是甘露糖结合的识别位点。
