Dipartimento di Scienze Chimiche, University of Catania, Viale A. Doria 6, 95125 Catania, Italy.
INSTM Udr of Catania, Catania 95125, Italy.
J Mater Chem B. 2024 Aug 14;12(32):7826-7836. doi: 10.1039/d4tb00651h.
The practical and easy detection of dopamine levels in human fluids, such as urine and saliva, is of great interest due to the correlation of dopamine concentration with several diseases. In this work, the one-step synthesis of water-soluble carbon nanoparticles (CNPs), starting from artichoke extract, containing catechol groups, for the fluorescence sensing of dopamine is reported. Size, morphology, chemical composition and electronic structure of CNPs were elucidated by DLS, AFM, XPS, FT-IR, EDX and TEM analyses. Their optical properties were then explored by UV-vis and fluorescence measurements in water. The dopamine recognition properties of these CNPs were investigated in water through fluorescence measurements and we observed the progressive enhancement of the CNP emission intensity upon the progressive addition of dopamine, with a binding affinity value of log = 5.76 and a detection limit of 0.81 nM. Selectivity towards dopamine was tested over other interfering analytes commonly present in human saliva. Finally, in order to perform a solid point of care test, CNPs were adsorbed on a solid support and exposed to different concentrations of dopamine, thus observing a pseudo-linear response, using a smartphone as a detector. Therefore, the detection of dopamine in simulated human saliva was performed with excellent results, in terms of selectivity and a detection limit of 100 pM.
由于多巴胺浓度与多种疾病相关，因此在人体体液（如尿液和唾液）中实际且便捷地检测多巴胺水平引起了广泛关注。在这项工作中，我们报道了从含有儿茶酚基团的朝鲜蓟提取物出发，一步合成水溶性碳纳米粒子（CNP），用于多巴胺的荧光传感。通过 DLS、AFM、XPS、FT-IR、EDX 和 TEM 分析阐明了 CNP 的尺寸、形态、化学成分和电子结构。然后通过在水中进行的紫外-可见和荧光测量来研究它们的光学性质。通过荧光测量研究了这些 CNP 在水中对多巴胺的识别特性，我们观察到随着多巴胺的逐渐加入，CNP 的发射强度逐渐增强，结合亲和力值为 log = 5.76，检测限为 0.81 nM。对其他常见于人体唾液中的干扰分析物进行了选择性测试。最后，为了进行现场即时检测，将 CNP 吸附在固体载体上并暴露于不同浓度的多巴胺中，从而使用智能手机作为检测器观察到伪线性响应。因此，使用该方法对模拟人唾液中的多巴胺进行了检测，具有出色的选择性和 100 pM 的检测限。
