College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China.
Shaoxing Customs, Shaoxing 312000, China.
Molecules. 2022 Nov 7;27(21):7623. doi: 10.3390/molecules27217623.
Niclosamide (NI) is the main molluscicide used to control (Lamarck) (Architaenioglossa: Ampullariidae). However, NI failed to inhibit snail climbing during the treatment process. In this study, we examined the effect of NI combined with pedunsaponin A at an ineffective concentration. The molluscicidal effect of Pedunsaponin A on NI was evidently synergistic after 48 h, and the synergism ratio (SR) was 1.82 after treatment for 72 h at 0.8 mg·L. Examination of the climbing adhesion effect showed that a high concentration of Pedunsaponin A (0.4 mg·L and 0.8 mg·L) combined with NI significantly inhibited the climbing of . We further studied the synergism mechanism; the results of histopathological observation showed that the siphon appeared cavities, the muscle fibers of the ventricular were severely dissolved, and kidney tubule arrangement was distorted after NI adding Pedunsaponin A. In addition, the hemocyte survival rate and the content of hemocyanin decreased significantly. According to the results of our study, the synergism mechanism may hinder oxygen transport of , influencing the supply of energy; the ability of immune defense and excretion and metabolic detoxification decreased, prolonging the action time of NI in the body.
硝氯酚 (NI) 是用于控制 (拉马克) (Architaenioglossa: Ampullariidae) 的主要杀螺剂。然而，NI 在处理过程中未能抑制蜗牛的攀爬。在这项研究中，我们研究了在无效浓度下与白头翁皂苷 A 联合使用 NI 的效果。48 h 后，白头翁皂苷 A 对 NI 的杀螺效果明显协同，72 h 后协同比（SR）为 0.8 mg·L 时为 1.82。对攀爬黏附效果的检查表明，高浓度的白头翁皂苷 A（0.4 mg·L 和 0.8 mg·L）与 NI 联合使用可显著抑制 的攀爬。我们进一步研究了协同作用机制；组织病理学观察结果表明，NI 加白头翁皂苷 A 后，虹吸管出现空洞，心室肌纤维严重溶解，肾小管排列扭曲。此外，血淋巴细胞存活率和血蓝蛋白含量显著降低。根据我们的研究结果，协同作用机制可能会阻碍 的氧气运输，影响能量供应；免疫防御和排泄以及代谢解毒能力下降，延长了 NI 在体内的作用时间。
