Kukushkin Vladimir, Ambartsumyan Oganes, Astrakhantseva Anna, Gushchin Vladimir, Nikonova Alexandra, Dorofeeva Anastasia, Zverev Vitaly, Gambaryan Alexandra, Tikhonova Daria, Sovetnikov Timofei, Akhmetova Assel, Yaminsky Igor, Zavyalova Elena
Osipyan Institute of Solid State Physics of Russian Academy of Science, 142432 Chernogolovka, Russia.
Department of Microbiology, Virology and Immunology, I.M. Sechenov First Moscow State Medical University, 125009 Moscow, Russia.
Nanomaterials (Basel). 2022 Nov 1;12(21):3854. doi: 10.3390/nano12213854.
In this paper, we propose a technology for the rapid and sensitive detection of the whole viral particles of SARS-CoV-2 using double-labeled DNA aptamers as recognition elements together with the SERS method for detecting the optical response. We report on the development of a SERS-aptasensor based on a reproducible lithographic SERS substrate, featuring the combination of high speed, specificity, and ultrasensitive quantitative detection of SARS-CoV-2 virions. The sensor makes it possible to identify SARS-CoV-2 in very low concentrations (the limit of detection was 100 copies/mL), demonstrating a sensitivity level comparable to the existing diagnostic golden standard-the reverse transcription polymerase chain reaction.
在本文中，我们提出了一种技术，该技术使用双标记DNA适配体作为识别元件，并结合表面增强拉曼散射（SERS）方法来检测光学响应，从而对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的完整病毒颗粒进行快速灵敏的检测。我们报告了一种基于可重现光刻SERS基底的SERS适配体传感器的开发情况，该传感器具有高速、特异性以及对SARS-CoV-2病毒粒子进行超灵敏定量检测的特点。该传感器能够识别极低浓度的SARS-CoV-2（检测限为100拷贝/毫升），其灵敏度水平与现有的诊断金标准——逆转录聚合酶链反应相当。
