Department of Chemistry, Chung-Ang University, Seoul 06974, South Korea.
Advanced Nano-Surface Department, Korea Institute of Materials Science (KIMS), Changwon 51508, South Korea.
ACS Sens. 2021 Jun 25;6(6):2378-2385. doi: 10.1021/acssensors.1c00596. Epub 2021 May 21.
We developed a new surface-enhanced Raman scattering (SERS)-based aptasensor platform capable of quantifying severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) lysates with a high sensitivity. In this study, a spike protein deoxyribonucleic acid (DNA) aptamer was used as a receptor, and a self-grown Au nanopopcorn surface was used as a SERS detection substrate for the sensible detection of SARS-CoV-2. A quantitative analysis of the SARS-CoV-2 lysate was performed by monitoring the change in the SERS peak intensity caused by the new binding between the aptamer DNA released from the Au nanopopcorn surface and the spike protein in the SARS-CoV-2 virion. This technique enables detecting SARS-CoV-2 with a limit of detection (LoD) of less than 10 PFU/mL within 15 min. The results of this study demonstrate the possibility of a clinical application that can dramatically improve the detection limit and accuracy of the currently commercialized SARS-CoV-2 immunodiagnostic kit.
我们开发了一种新的基于表面增强拉曼散射（SERS）的适体传感器平台，能够高度灵敏地定量检测严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）裂解物。在这项研究中，一种刺突蛋白 DNA 适体被用作受体，并且自生长的 Au 纳米爆米花表面被用作 SERS 检测基底，用于 SARS-CoV-2 的灵敏检测。通过监测从 Au 纳米爆米花表面释放的适体 DNA 与 SARS-CoV-2 病毒粒子中的刺突蛋白之间新结合引起的 SERS 峰强度的变化，对 SARS-CoV-2 裂解物进行定量分析。该技术能够在 15 分钟内检测到 SARS-CoV-2 的检测限（LoD）低于 10PFU/mL。这项研究的结果表明了一种临床应用的可能性，该应用可以极大地提高目前商业化 SARS-CoV-2 免疫诊断试剂盒的检测限和准确性。
