The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs), The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao 266003, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao 266237, China.
Nutrients. 2022 Oct 26;14(21):4504. doi: 10.3390/nu14214504.
The early life period is considered an essential period for gut microbial colonization. Manipulating gut microbiota interventions during early life periods has been proven to be a promising method to boost healthy growth. Therefore, the aim of the present study was to investigate the effects of dietary fucoidan (Fuc) on the growth, digestive tract maturation, and gut microbiota of large yellow croaker () larvae. Four diets were formulated with different levels of Fuc (0.00%, 0.50%, 1.00%, and 2.00%). Results showed that dietary Fuc significantly improved the growth performance of larvae. Meanwhile, dietary Fuc promoted digestive tract maturation. Dietary 1.00% Fuc significantly improved intestinal morphology. Dietary Fuc upregulated the expression of intestinal cell proliferation and differentiation related-genes and intestinal barrier related-genes. Dietary 2.00% Fuc significantly increased the activities of brush border membranes enzymes and lipase while inhibiting α-amylase. Furthermore, dietary Fuc maintained healthy intestinal micro-ecology. In detail, dietary 1.00% and 2.00% Fuc altered the overall structure of the gut microbiota and increased the relative abundance of Bacteroidetes while decreasing the relative abundance of opportunistic pathogens and facultative anaerobe. In conclusion, appropriate dietary Fuc (1.00-2.00%) could improve the growth of large yellow croaker larvae by promoting digestive tract maturation and maintaining an ideal intestinal micro-ecology.
生命早期被认为是肠道微生物定植的关键时期。在生命早期阶段操纵肠道微生物群干预已被证明是促进健康生长的有前途的方法。因此，本研究旨在探讨饲料褐藻糖胶（Fuc）对大黄鱼（）幼虫生长、消化道成熟和肠道微生物群的影响。用不同水平的 Fuc（0.00%、0.50%、1.00%和 2.00%）配制了四种饲料。结果表明，饲料 Fuc 显著提高了幼虫的生长性能。同时，饲料 Fuc 促进了消化道成熟。饲料中添加 1.00%的 Fuc 可显著改善肠道形态。饲料 Fuc 上调了肠道细胞增殖和分化相关基因和肠道屏障相关基因的表达。饲料 Fuc 增加了肠刷状缘膜酶和脂肪酶的活性，同时抑制了α-淀粉酶的活性。此外，饲料 Fuc 维持了健康的肠道微生态。具体而言，饲料 Fuc（1.00%和 2.00%）改变了肠道微生物群的整体结构，增加了厚壁菌门的相对丰度，同时降低了机会致病菌和兼性厌氧菌的相对丰度。总之，适当的饲料 Fuc（1.00-2.00%）可以通过促进消化道成熟和维持理想的肠道微生态来提高大黄鱼幼虫的生长。
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