CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Av. Infante D. Henrique, 6200-506 Covilhã, Portugal.
CNC-Centre for Neuroscience and Cell Biology, Faculty of Medicine, University of Coimbra, 3004-504 Coimbra, Portugal.
Nutrients. 2022 Oct 31;14(21):4576. doi: 10.3390/nu14214576.
The bioactivity of natural by-products in food and pharmaceutical applications is the subject of numerous studies. Cherry production and processing generates large amounts of biowaste, most of which is not used. The recovery of these by-products is essential for promoting the circular economy and to improving sustainability in the food industry. In this work, we explored the anti-inflammatory and antimicrobial potential of two different extracts from stems, leaves, and flowers of Portuguese cherries. The anti-inflammatory potential was studied on lipopolysaccharide (LPS)-stimulated mouse macrophages (RAW 264.7) by evaluating the effect of by-products on cellular viability and nitric oxide (NO) production. Disc diffusion and minimum inhibitory concentration (MIC) were used to determine antimicrobial activity. The cherry by-products had no cytotoxic effect on RAW 264.7 cells, and were able to inhibit nitrite production in a dose-dependent manner. Moreover, all aqueous infusions showed good antioxidant activity against NO radicals. Moreover, leaf extracts showed the best activity against most of the strains studied. The results revealed, for the first time, interesting anti-inflammatory and antimicrobial properties of cherry by-products. This could potentially be of interest for their therapeutic use in the treatment of inflammation-related diseases or in controlling the growth of microorganisms.
食品和制药应用中天然副产品的生物活性是众多研究的主题。樱桃的生产和加工会产生大量的生物废料，其中大部分未被利用。回收这些副产品对于促进循环经济和提高食品工业的可持续性至关重要。在这项工作中，我们探索了来自葡萄牙樱桃的茎、叶和花的两种不同提取物的抗炎和抗菌潜力。通过评估副产品对细胞活力和一氧化氮 (NO) 产生的影响，研究了抗炎潜力在脂多糖 (LPS) 刺激的小鼠巨噬细胞 (RAW 264.7) 上的作用。采用圆盘扩散法和最小抑菌浓度 (MIC) 测定抗菌活性。樱桃副产品对 RAW 264.7 细胞没有细胞毒性作用，并能够以剂量依赖的方式抑制亚硝酸盐的产生。此外，所有水提物均显示出良好的抗 NO 自由基活性。此外，叶提取物对大多数研究的菌株表现出最佳的活性。研究结果首次揭示了樱桃副产品的有趣的抗炎和抗菌特性。这对于它们在治疗炎症相关疾病或控制微生物生长方面的治疗用途可能具有潜在的意义。
