Institute of Plant Breeding and Genetic Resources, ELGO-DEMETER, 57001, Thessaloniki-Thermi, Greece.
Institute of Olive Tree, Subtropical Crops and Viticulture (IOSV), Department of Vitis, ELGO-DEMETER, Lykovrysi, 14123, Athens, Greece.
Mol Biol Rep. 2022 Apr;49(4):2687-2693. doi: 10.1007/s11033-021-07076-4. Epub 2022 Jan 16.
Natural products are not only positioned in the heart of traditional medicine but also in modern medicine as many current drugs are coming from natural sources. Apart from the field of medicine and therapeutics, natural products are broadly used in other industrial fields such as nutrition, skincare products and nanotechnology.
The aim of this study was to assess the effects of sweet cherry (Prunus avium L.) fruit extract from the Greek native cultivar 'Vasiliadi', on the human 2D and 3D in vitro models in order to investigate its potential impact on skin. We focused on 2D culture of primary normal human epidermal keratinocytes (NHEK) that were treated with sweet cherry fruit extract. In the first place, we targeted fruit extract potential cytotoxicity by determining ATP intracellular levels. Furthermore, we assessed its potential skin irritability by using 3D skin model. To better understand the bioactivity of sweet cherry fruit. extract, we used qPCR to study the expression of various genes that are implicated in the skin functions. Our experiments showed that sweet cherry fruit extract is non-toxic in 2D keratinocytes culture as well as non-irritant in 3D skin model. Our results revealed that the extract mediated important pathways for the optimum epidermis function such as cell proliferation, immune and inflammatory response.
The sweet cherry fruit extracts possesses significant activity in epidermis function without any potential of cytotoxicity or skin irritability, which makes it a rather promising active agent for skincare.
天然产物不仅是传统医学的核心，而且在现代医学中也有许多当前的药物来自天然来源。除了医学和治疗领域，天然产物在营养、护肤品和纳米技术等其他工业领域也得到广泛应用。
本研究的目的是评估希腊本地品种“Vasiliadi”甜樱桃（Prunus avium L.）果实提取物对人体 2D 和 3D 体外模型的影响，以研究其对皮肤的潜在影响。我们专注于用甜樱桃果实提取物处理的原代正常人表皮角质形成细胞（NHEK）的 2D 培养。首先，我们通过测定细胞内 ATP 水平来确定果实提取物的潜在细胞毒性。此外，我们使用 3D 皮肤模型评估其潜在的皮肤刺激性。为了更好地理解甜樱桃果实提取物的生物活性，我们使用 qPCR 研究了与皮肤功能相关的各种基因的表达。我们的实验表明，甜樱桃果实提取物在 2D 角质形成细胞培养中无毒性，在 3D 皮肤模型中也无刺激性。我们的结果表明，该提取物介导了表皮功能的重要途径，如细胞增殖、免疫和炎症反应。
甜樱桃果实提取物在不具有细胞毒性或皮肤刺激性的情况下对表皮功能具有显著的活性，使其成为一种有前途的护肤品活性成分。
