Department of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Korea.
National Development Institute of Korean Medicine, Gyeongsan-si 38540, Gyeongsangbuk-do, Korea.
Nutrients. 2022 Nov 5;14(21):4683. doi: 10.3390/nu14214683.
Akebia quinata, commonly called chocolate vine, has various bioactivities, including antioxidant and anti-obesity properties. However, the anti-obesity effects of bioconverted extracts of A. quinate have not been examined. In this study, A. quinata fruit extracts was bioconverted using the enzyme isolated from the soybean paste fungi Aspergillus kawachii. To determine whether the bioconversion process could influence the anti-obesity effects of A. quinata fruit extracts, we employed 3T3-L1 adipocytes and HFD-induced obese rats. We observed that the bioconverted fruit extract of A. quinata (BFE) afforded anti-obesity effects, which were stronger than that for the non-bioconverted fruit extract (FE) of A. quinata. In 3T3-L1 adipocytes, treatment with BFE at concentrations of 20 and 40 μg reduced intracellular lipids by 74.8 (p < 0.05) and 54.9% (p < 0.01), respectively, without inducing cytotoxicity in preadipocytes. Moreover, the oral administration of BFE at the concentration of 300 mg/kg/day significantly reduced body and adipose tissue weights (p < 0.01) in HFD-induced obese rats. Plasma cholesterol values were reduced, whereas HDL was increased in BFE receiving rats. Although FE could exert anti-obesity effects, BFE supplementation induced more robust effects than FE. These results could be attributed to the bioconversion-induced alteration of bioactive compound content within the extract.
三叶木通，俗称八月炸，具有多种生物活性，包括抗氧化和抗肥胖特性。然而，三叶木通生物转化提取物的抗肥胖作用尚未得到检验。在这项研究中，使用从豆酱真菌米曲霉中分离出的酶对三叶木通果实提取物进行了生物转化。为了确定生物转化过程是否会影响三叶木通果实提取物的抗肥胖作用，我们使用了 3T3-L1 脂肪细胞和高脂肪饮食诱导的肥胖大鼠。我们观察到，三叶木通的生物转化果实提取物（BFE）具有抗肥胖作用，其作用强于三叶木通的非生物转化果实提取物（FE）。在 3T3-L1 脂肪细胞中，BFE 浓度为 20 和 40 μg 时，分别使细胞内脂质减少了 74.8%（p<0.05）和 54.9%（p<0.01），而对前脂肪细胞没有细胞毒性。此外，BFE 的口服剂量为 300 mg/kg/天时，可显著降低高脂肪饮食诱导的肥胖大鼠的体重和脂肪组织重量（p<0.01）。BFE 组的血浆胆固醇值降低，而高密度脂蛋白升高。虽然 FE 可以发挥抗肥胖作用，但 BFE 的补充作用比 FE 更有效。这些结果可能归因于生物转化诱导的提取物中生物活性化合物含量的改变。
