Rattis Bruna A C, Piva Henrique L, Duarte Andressa, Gomes Frederico G F L R, Lellis Janaína R, Soave Danilo F, Ramos Simone G, Tedesco Antonio C, Celes Mara R N
Department of Pathology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-900, São Paulo, Brazil.
Department of Bioscience and Technology, Institute of Tropical Pathology and Public Health, Federal University of Goias, Goiânia 74605-050, Goias, Brazil.
Pharmaceutics. 2022 Oct 24;14(11):2277. doi: 10.3390/pharmaceutics14112277.
mTOR is a signaling pathway involved in cell survival, cell stress response, and protein synthesis that may be a key point in sepsis-induced cardiac dysfunction. Curcumin has been reported in vitro as an mTOR inhibitor compound; however, there are no studies demonstrating this effect in experimental sepsis. Thus, this study aimed to evaluate the action of curcumin on the mTOR pathway in the heart of septic mice. Free curcumin (FC) and nanocurcumin (NC) were used, and samples were obtained at 24 and 120 h after sepsis. Histopathological and ultrastructural analysis showed that treatments with FC and NC reduced cardiac lesions caused by sepsis. Our main results demonstrated that curcumin reduced mTORC1 and Raptor mRNA at 24 and 120 h compared with the septic group; in contrast, mTORC2 mRNA increased at 24 h. Additionally, the total mTOR mRNA expression was reduced at 24 h compared with the septic group. Our results indicate that treatment with curcumin and nanocurcumin promoted a cardioprotective response that could be related to the modulation of the mTOR pathway.
mTOR是一条参与细胞存活、细胞应激反应和蛋白质合成的信号通路，可能是脓毒症诱导的心脏功能障碍的关键点。姜黄素在体外已被报道为一种mTOR抑制剂化合物；然而，尚无研究在实验性脓毒症中证实这种作用。因此，本研究旨在评估姜黄素对脓毒症小鼠心脏中mTOR通路的作用。使用了游离姜黄素（FC）和纳米姜黄素（NC），并在脓毒症后24小时和120小时获取样本。组织病理学和超微结构分析表明，FC和NC处理减少了脓毒症引起的心脏病变。我们的主要结果表明，与脓毒症组相比，姜黄素在24小时和120小时时降低了mTORC1和Raptor mRNA；相反，mTORC2 mRNA在24小时时增加。此外，与脓毒症组相比，总mTOR mRNA表达在24小时时降低。我们的结果表明，姜黄素和纳米姜黄素处理促进了一种心脏保护反应，这可能与mTOR通路的调节有关。
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