Kim Jung-Hwan, Yang Heejung, Kim Kee K
Department of Pharmacology, School of Medicine, Institute of Health Sciences, Gyeongsang National University, Jinju 52727, Korea.
Laboratory of Natural Products Chemistry, College of Pharmacy, Kangwon National University, Chuncheon 24341, Korea.
Plants (Basel). 2022 Oct 29;11(21):2914. doi: 10.3390/plants11212914.
L. (Theaceae) has been used for medicinal and cosmetic purposes in East Asian countries. Most functional components were obtained from the upper parts of the tree, such as leaves, flowers, or seeds. Here, we report a functional effect of the 80% methanolic extract of root (CJRE) on antioxidative stress in HeLa cells. The nuclear factor erythroid-derived 2-related factor 2 (NRF2) is a key transcription factor that triggers the induction of oxidative stress-relating genes and drug detoxification. As result, CJRE showed a strong anti-radical scavenging effect in a dose-dependent manner. In addition, the induction of antioxidant response elements (ARE)-luciferase activity was maximized at CJRE 200 µg/mL. Furthermore, CJRE induced the mRNA levels of and by the nuclear NRF2 accumulation. As a possible mechanism of Nrf2 activation, the phosphorylation of p38 and ERK1/2 signaling might fortify the NRF2 induction as well as its stability. However, the phosphorylation of AKT is rather decreased. Taken together, CJRE may potentiate the antioxidant effects by increasing the NRF2 signaling through MAP kinase signaling and the properties of its radical scavenging activity. Thus, CJRE could apply for other medicinal and cosmetic purposes.
L.（山茶科）在东亚国家已被用于医药和化妆品用途。大多数功能成分取自树的上部，如叶子、花朵或种子。在此，我们报告了根的80%甲醇提取物（CJRE）对HeLa细胞抗氧化应激的功能作用。核因子红细胞衍生2相关因子2（NRF2）是一种关键转录因子，可触发氧化应激相关基因的诱导和药物解毒。结果，CJRE呈剂量依赖性地表现出强大的抗自由基清除作用。此外，抗氧化反应元件（ARE）-荧光素酶活性在CJRE 200 µg/mL时达到最大值。此外，CJRE通过核NRF2积累诱导了[具体基因1]和[具体基因2]的mRNA水平。作为Nrf2激活的一种可能机制，p38和ERK1/2信号的磷酸化可能会加强NRF2的诱导及其稳定性。然而，AKT的磷酸化反而降低。综上所述，CJRE可能通过丝裂原活化蛋白激酶信号增加NRF2信号及其自由基清除活性的特性来增强抗氧化作用。因此，CJRE可用于其他医药和化妆品用途。
