Janampa-Sarmiento Peter Charrie, Costa Henrique Lopes, Rosa Júlio César Câmara, Tavares Guilherme Campos, Figueiredo Henrique César Pereira
Laboratory of Aquatic Animal Diseases-Aquavet, Department of Preventive Veterinary Medicine, School of Veterinary Medicine, Federal University of Minas Gerais-UFMG, Belo Horizonte 31270-901, MG, Brazil.
Pathogens. 2025 Feb 22;14(3):219. doi: 10.3390/pathogens14030219.
Due to the recent taxonomic reclassification of the species into four new species-, , , and -it is necessary to re-evaluate isolates of previous outbreaks to better understand the epidemiology related to this bacterial group. Therefore, the aim of this study was to determine the taxonomic profile of Brazilian isolates of spp. associated with columnaris disease using available diagnostic methods. Fifty isolates from different outbreaks (17 clinical cases) occurring in five different Brazilian states previously identified as were selected and identified by multiplex PCR and MALDI-ToF methods. In addition, at least one isolate from each clinical case was analyzed by gene sequencing. After inclusion of the MSPs (main spectra profiles), the isolates were identifiable, and when compared with the multiplex PCR results, they showed almost perfect agreement (94.2% Kappa = 0.85). Only , , and were found among the Brazilian isolates, with these species causing disease in neotropical fish hosts not previously reported (e.g., Siluriformes, Serrasalmidae, and Bryconidae), while was not detected. This study provides evidence of species associated with columnaris disease circulating in various aquaculture facilities across different regions of Brazil. This information is crucial for developing control programs and advancing epidemiologic studies on columnaris disease in Brazilian aquaculture.
由于该物种最近被重新分类为四个新物种——[具体物种1]、[具体物种2]、[具体物种3]和[具体物种4]，有必要对先前疫情的分离株进行重新评估，以更好地了解与该细菌群相关的流行病学情况。因此，本研究的目的是使用现有的诊断方法确定巴西分离的[物种名称] spp. 与柱状病相关的分类学特征。从巴西五个不同州发生的不同疫情（17例临床病例）中选取了50株先前鉴定为[物种名称]的分离株，并通过多重PCR和基质辅助激光解吸电离飞行时间质谱（MALDI-ToF）方法进行鉴定。此外，对每个临床病例中至少一株分离株进行[基因名称]基因测序分析。纳入主要光谱图谱（MSPs）后，这些分离株是可识别的，并且与多重PCR结果相比，它们显示出几乎完全一致（94.2%，卡帕值 = 0.85）。在巴西分离株中仅发现了[具体物种1]、[具体物种2]和[具体物种3]，这些物种在新热带鱼类宿主中引发疾病，此前未有报道（例如，鲶形目、锯脂鲤科和脂鲤科），而未检测到[具体物种4]。本研究提供了与柱状病相关的[物种名称]物种在巴西不同地区的各种水产养殖设施中传播的证据。这些信息对于制定控制计划和推进巴西水产养殖中柱状病的流行病学研究至关重要。
