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自适应照明傅里叶叠层显微术所需成像数据量及图像重建时间的减少

Reduction in required volume of imaging data and image reconstruction time for adaptive-illumination Fourier ptychographic microscopy.

作者信息

Luo Jiaxiong, Tan Haishu, Wu Ruofei, Zhu Sicong, Chen Hanbao, Zhen Junrui, Li Jiancong, Guan Caizhong, Wu Yanxiong

机构信息

School of Physics and Optoelectronic Engineering, Foshan University, Foshan, China.

Ji Hua Laboratory, Foshan, China.

出版信息

J Biophotonics. 2023 Mar;16(3):e202200240. doi: 10.1002/jbio.202200240. Epub 2022 Nov 14.

Abstract

Fourier ptychographic microscopy (FPM) is a promising super-resolution computational imaging technology. It stitches a series of low-resolution (LR) images in the Fourier domain by an iterative method. Thus, it obtains a large field of view and high-resolution quantitative phase images. Owing to its capability to perform high-spatial bandwidth product imaging, FPM is widely used in the reconstruction of conventional static samples. However, the influence of the FPM imaging mechanism limits its application in high-speed dynamic imaging. To solve this problem, an adaptive-illumination FPM scheme using regional energy estimation is proposed. Starting with several captured real LR images, the energy distribution of all LR images is estimated, and select the measurement images with large information to perform FPM reconstruction. Simulation and experimental results show that the method produces efficient imaging performance and reduces the required volume of data to more than 65% while ensuring the quality of FPM reconstruction.

摘要

傅里叶叠层显微镜(FPM)是一种很有前景的超分辨率计算成像技术。它通过迭代方法在傅里叶域中拼接一系列低分辨率(LR)图像。因此,它可以获得大视场和高分辨率的定量相位图像。由于其具有执行高空间带宽积成像的能力,FPM被广泛应用于传统静态样本的重建。然而,FPM成像机制的影响限制了其在高速动态成像中的应用。为了解决这个问题,提出了一种使用区域能量估计的自适应照明FPM方案。从几个捕获的真实LR图像开始,估计所有LR图像的能量分布,并选择具有大量信息的测量图像进行FPM重建。仿真和实验结果表明,该方法具有高效的成像性能,在确保FPM重建质量的同时,将所需的数据量减少到65%以上。

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