Opt Lett. 2021 Oct 1;46(19):4785-4788. doi: 10.1364/OL.428731.
Fourier ptychographic microscopy (FPM), as an emerging computational imaging method, has been applied to quantitative phase imaging with resolution bypassing the physical limit of the detection objective. Due to the weak illumination intensity and long image acquisition time, the achieved imaging speed in current FPM methods is still low, making them unsuitable for real-time imaging applications. We propose and demonstrate a high-speed FPM method based on using laser illumination and digital micro-mirror devices for illumination angle scanning. In this new, to the best of our knowledge, FPM method, we realized quantitative phase imaging and intensity imaging at over 42 frames per second (fps) with around 1 µm lateral resolution. The quantitative phase images have revealed membrane height fluctuations of red blood cells with nanometer-scale sensitivity, while the intensity images have resolved subcellular features in stained cancer tissue slices.
傅里叶叠层相位显微镜（FPM）作为一种新兴的计算成像方法，已经被应用于定量相位成像，其分辨率突破了检测物镜的物理极限。由于弱光照明强度和长的图像采集时间，当前 FPM 方法的成像速度仍然较低，使得它们不适合实时成像应用。我们提出并演示了一种基于激光照明和数字微镜器件的照明角度扫描的高速 FPM 方法。在这个新的 FPM 方法中，据我们所知，我们实现了定量相位成像和强度成像，帧率超过 42 帧每秒（fps），具有约 1 µm 的横向分辨率。定量相位图像揭示了红细胞的膜高度波动，具有纳米级的灵敏度，而强度图像则解析了染色的癌症组织切片中的亚细胞特征。
