原发性抗磷脂综合征患者单核细胞中lncRNAs的失调：一种生物信息学和实验性概念验证方法。
lncRNAs dysregulation in monocytes from primary antiphospholipid syndrome patients: a bioinformatic and an experimental proof-of-concept approach.
作者信息
Guzmán-Martín Carlos A, Juárez-Vicuña Yaneli, Domínguez-López Aarón, González-Ramírez Javier, Amezcua-Guerra Luis M, Martínez-Martínez Laura A, Sánchez-Muñoz Fausto
机构信息
Maestría en Ciencias de la Salud, Posgrado de la Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico City, Mexico.
Departamento de Inmunología, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Sección XVI, Tlalpan, 14080, Mexico City, Mexico.
出版信息
Mol Biol Rep. 2023 Jan;50(1):937-941. doi: 10.1007/s11033-022-08080-y. Epub 2022 Nov 11.
BACKGROUND
Antiphospholipid syndrome (APS) is the main cause of acquired thrombophilia where peripheral circulating cells such as monocytes have a key role. Currently, several studies have linked long non-coding RNAs (lncRNAs) in different inflammatory and autoimmune processes, including lupus. However, the role of lncRNAs in antiphospholipid syndrome is unknown, therefore, we aimed to select and measure expression levels of three lncRNAs based on its abundance in monocytes from APS patients.
METHODS
Selection of lncRNAs candidates were carried out based on its abundance in monocytes and their relationship with Perez-Sanchez miRNA signature by using miRNet 2.0 bioinformatic tool, then lncRNAs expression levels was measured in monocytes by RT-qPCR.
RESULTS
This is the first study to report that lncRNAs: FGD5-AS1, OIP5-AS1 and GAS5 are promising candidates for play a role on APS monocytes and they are expressed differently between patients and controls.
CONCLUSIONS
OIP5-AS1, FGD5-AS1 and GAS5 are downregulated on monocytes from APS patients.
背景
抗磷脂综合征（APS）是获得性血栓形成倾向的主要原因，其中外周循环细胞如单核细胞起关键作用。目前，多项研究已将长链非编码RNA（lncRNA）与包括狼疮在内的不同炎症和自身免疫过程联系起来。然而，lncRNA在抗磷脂综合征中的作用尚不清楚，因此，我们旨在根据APS患者单核细胞中的丰度选择并测量三种lncRNA的表达水平。
方法
通过使用miRNet 2.0生物信息学工具，基于lncRNA在单核细胞中的丰度及其与佩雷斯 - 桑切斯miRNA特征的关系来选择lncRNA候选物，然后通过RT-qPCR测量单核细胞中lncRNA的表达水平。
结果
这是第一项报告lncRNA：FGD5-AS1、OIP5-AS1和GAS5有望在APS单核细胞中发挥作用且在患者和对照之间表达不同的研究。
结论
OIP5-AS1、FGD5-AS1和GAS5在APS患者的单核细胞中表达下调。
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