Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Exp Mol Pathol. 2020 Apr;113:104383. doi: 10.1016/j.yexmp.2020.104383. Epub 2020 Jan 23.
Long non-coding RNAs (lncRNAs) have several important functions in the regulation of cell homeostasis and cell fate. Consequently, abnormal transcription of lncRNAs has been correlated with malignant transformation of cells. These human transcripts have been shown to participate in the progression of gastric cancer.
In the current project, we evaluated expression of a panel of lncRNAs including HULC, MALAT1, FAS-AS1, GAS5, PVT1, OIP5-AS1 and THRIL in 30 gastric cancer tissues and paired adjacent non-cancerous tissues (ANCTs) using quantitative real-time PCR.
HULC, OIP5-AS1 and THRIL transcription quantities were significantly lower in gastric tumors compared to ANCTs (P values = .02, 0.02 and 0.007, respectively). Relative transcription quantities of HULC, MALAT1, OIP5-AS1, PVT1, FAS-AS1 and THRIL were associated with the site of the primary tumor (P values = .002, 0.003, 0.002, 0.002, 0.002, and 0.001, respectively). Moreover, relative expression levels of PVT1 were associated with history of smoking (P value = .04). Correlations were identified between transcript quantities of these lncRNAs in both tumor samples and ANCTs. Receiver operating characteristic curve assessment demonstrated that THRIL had the highest diagnostic power among the mentioned lncRNAs (area under curve (AUC) = 0.72, P value = .001). HULC and OIP5-AS1 ranked afterwards (AUC values of 0.69 and 0.68; P values = .005 and 0.007, respectively).
The current investigation underscores the dysregulation of these transcripts in gastric cancer specimens and suggests a number of these transcripts for further assessments of their suitability as cancer biomarkers.
长链非编码 RNA（lncRNA）在细胞内稳态和细胞命运的调节中具有多种重要功能。因此，lncRNA 的转录异常与细胞的恶性转化有关。这些人类转录物已被证明参与了胃癌的进展。
在目前的项目中，我们使用定量实时 PCR 评估了 30 例胃癌组织和配对的癌旁非肿瘤组织（ANCT）中一组 lncRNA 的表达情况，包括 HULC、MALAT1、FAS-AS1、GAS5、PVT1、OIP5-AS1 和 THRIL。
与 ANCT 相比，胃癌肿瘤中 HULC、OIP5-AS1 和 THRIL 的转录量明显降低（P 值分别为 0.02、0.02 和 0.007）。HULC、MALAT1、OIP5-AS1、PVT1、FAS-AS1 和 THRIL 的相对转录量与原发性肿瘤的部位有关（P 值分别为 0.002、0.003、0.002、0.002、0.002 和 0.001）。此外，PVT1 的相对表达水平与吸烟史有关（P 值为 0.04）。在肿瘤样本和 ANCT 中都发现了这些 lncRNA 的转录物之间的相关性。受试者工作特征曲线评估表明，THRIL 在所述 lncRNA 中具有最高的诊断能力（曲线下面积（AUC）为 0.72，P 值为 0.001）。HULC 和 OIP5-AS1 紧随其后（AUC 值分别为 0.69 和 0.68；P 值分别为 0.005 和 0.007）。
目前的研究强调了这些转录物在胃癌标本中的失调，并提出了其中一些转录物进一步评估其作为癌症生物标志物的适用性。
