Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, PR China.
College of Physical Education and Sports Rehabilitation, Jinzhou Medical University, Jinzhou, PR China.
Pharm Biol. 2022 Dec;60(1):2229-2236. doi: 10.1080/13880209.2022.2140168.
Ginsenoside Rb1 (Rb1) is a biologically active component of ginseng [ C.A. Meyer (Araliaceae)].
This study determined the underlying mechanisms of Rb1 treatment that acted on diabetes-injured lungs in diabetic rats.
Streptozotocin (STZ)-induced diabetic rat model was used. Male Sprague-Dawley (SD) rats were divided into four groups ( = 10): control, Rb1 (20 mg/kg), insulin (15 U/kg to attain the euglycaemic state) and diabetic (untreated). After treatment for six weeks, oxidative stress assay; histological and ultrastructure analyses; TNF-α, TGF-β, IL-1 and IL-6 protein expression analyses; and the detection of apoptosis were performed.
There was decreased activity of SOD (3.53-fold), CAT (2.55-fold) and GSH (1.63-fold) and increased levels of NO (4.47-fold) and MDA (3.86-fold) in the diabetic group from control. Rb1 treatment increased SOD (2.4-fold), CAT (1.9-fold) and GSH (1.29-fold) and decreased the levels of NO (1.76-fold) and MDA (1.51-fold) as compared with diabetic rats. The expression of IL-6 (5.13-fold), IL-1α (2.35-fold), TNF-α (2.35-fold) and TGF-β (2.39-fold) was increased in diabetic rats from control. IL-6 (2.43-fold), IL-1α (2.27-fold), TNF-α (1.68-fold) and TGF-β (2.3-fold) were decreased in the Rb1 treatment group. Diabetes increased the apoptosis rate (2.23-fold vs. control), and Rb1 treatment decreased the apoptosis rate (1.73-fold vs. the diabetic rats). Rb1 and insulin ameliorated lung tissue injury.
These findings indicate that Rb1 could be useful for mitigating oxidative damage and inflammatory infiltration in the diabetic lung.
人参中的生物活性成分人参皂苷 Rb1（Rb1）。
本研究旨在确定 Rb1 治疗糖尿病大鼠肺损伤的潜在机制。
采用链脲佐菌素（STZ）诱导的糖尿病大鼠模型。雄性 Sprague-Dawley（SD）大鼠分为四组（每组 10 只）：对照组、Rb1（20mg/kg）组、胰岛素（15U/kg 以达到正常血糖状态）组和糖尿病组（未治疗）。治疗 6 周后，进行氧化应激测定；组织学和超微结构分析；TNF-α、TGF-β、IL-1 和 IL-6 蛋白表达分析；以及凋亡检测。
与对照组相比，糖尿病组 SOD（3.53 倍）、CAT（2.55 倍）和 GSH（1.63 倍）活性降低，NO（4.47 倍）和 MDA（3.86 倍）水平升高。Rb1 治疗组 SOD（2.4 倍）、CAT（1.9 倍）和 GSH（1.29 倍）活性升高，NO（1.76 倍）和 MDA（1.51 倍）水平降低。与对照组相比，糖尿病大鼠 IL-6（5.13 倍）、IL-1α（2.35 倍）、TNF-α（2.35 倍）和 TGF-β（2.39 倍）表达增加。Rb1 治疗组 IL-6（2.43 倍）、IL-1α（2.27 倍）、TNF-α（1.68 倍）和 TGF-β（2.3 倍）表达降低。糖尿病大鼠的细胞凋亡率增加（与对照组相比增加 2.23 倍），Rb1 治疗组的细胞凋亡率降低（与糖尿病大鼠相比降低 1.73 倍）。Rb1 和胰岛素改善了肺组织损伤。
这些发现表明，Rb1 可能有助于减轻糖尿病肺的氧化损伤和炎症浸润。
