Tianjin Anding Hospital, Tianjian 300022, P. R. China.
Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, P. R. China.
Am J Chin Med. 2021;49(5):1195-1213. doi: 10.1142/S0192415X21500579. Epub 2021 May 27.
(Huang-lian) and Asian have been widely used in the treatment of diabetes and other concurrent diseases with apparent effects. This study investigated the effects of the active ingredients of and , berberine and ginsenoside Rb1, on depression-like behavior in a rat diabetes model. The animal model was established via a high-fat diet and intraperitoneal injection of streptozotocin, while the animal's depression-like behavior was induced via chronic unpredictable mild stress. These experimental rats were divided into four groups: control, depression-like behavior (DLB), metformin plus fluoxetine hydrochloride (M+FH), and berberine plus ginsenoside Rb1 (B+GRb1) groups. Glucose metabolism and insulin resistance were evaluated by oral glucose test and glucose clamp study. Depression-like behavior was evaluated via behavioral analyses, including forced swim, sucrose preference, elevated plus maze, and open-field tests. HE and Nissl staining, plasma cortisol expression of adrenocorticotropic hormone, and brain-derived neurotrophic factor (BDNF) levels were assayed to explore the mechanisms of action. Compared with the control, rats in the DLB group had a significant increase in the levels of blood glucose and depression-like behavior. The B+GRb1 group significantly improved glucose metabolism and insulin resistance, reduced depression-like behavior, downregulated levels of plasma cortisol and adrenocorticotropic hormone under stress, and upregulated BDNF protein expression compared to the DLB rats. HE and Nissl staining data revealed that B+GRb1 protected neurons from pathological and morphological changes. Thus, berberine and ginsenoside Rb1 not only improved glucose metabolism in diabetic rats but also ameliorated their depression-like behavior under chronic unpredictable stress. Mechanistically, studied data with plasma hormonal levels and brain neuronal pathological/morphological changes supported the observed effects. The combination of berberine and ginsenoside Rb1 may have a clinical value in the management of diabetic patients with depression.
黄连和亚洲人参被广泛用于治疗糖尿病和其他并发疾病，疗效显著。本研究探讨了黄连、小檗碱和人参皂苷 Rb1 的活性成分对糖尿病大鼠模型抑郁样行为的影响。采用高脂饮食和腹腔注射链脲佐菌素建立动物模型，采用慢性不可预测轻度应激诱导动物的抑郁样行为。将这些实验大鼠分为四组：对照组、抑郁样行为（DLB）组、二甲双胍加盐酸氟西汀（M+FH）组和小檗碱加人参皂苷 Rb1（B+GRb1）组。通过口服葡萄糖试验和葡萄糖钳夹研究评估葡萄糖代谢和胰岛素抵抗。通过行为分析评估抑郁样行为，包括强迫游泳、蔗糖偏好、高架十字迷宫和旷场试验。进行 HE 和尼氏染色、血浆促肾上腺皮质激素皮质醇表达和脑源性神经营养因子（BDNF）水平测定，以探讨作用机制。与对照组相比，DLB 组大鼠血糖和抑郁样行为明显升高。与 DLB 大鼠相比，B+GRb1 组显著改善了葡萄糖代谢和胰岛素抵抗，降低了抑郁样行为，应激下下调了血浆皮质醇和促肾上腺皮质激素水平，并上调了 BDNF 蛋白表达。HE 和尼氏染色数据显示，B+GRb1 可保护神经元免受病理和形态变化的影响。因此，小檗碱和人参皂苷 Rb1 不仅改善了糖尿病大鼠的葡萄糖代谢，而且改善了慢性不可预测应激下的抑郁样行为。研究数据结合血浆激素水平和脑神经元病理/形态变化支持观察到的作用。小檗碱和人参皂苷 Rb1 的联合应用可能在糖尿病伴抑郁患者的治疗中具有临床价值。
