Zhang Pengbo, Zhang Xibo, Xu Fen, Xu Wangdong, Zhu Huang
Department of Ophthalmology, Xinhua Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China.
Department of Ophthalmology, Affiliated Hospital of Southwest Medical University, Luzhou, 646000, Sichuan, China.
Graefes Arch Clin Exp Ophthalmol. 2023 Apr;261(4):1091-1100. doi: 10.1007/s00417-022-05856-7. Epub 2022 Nov 12.
Thyroid-associated ophthalmopathy (TAO) is a chronic autoimmune disease. The interleukin-12 (IL-12) family includes IL-12, IL-23, IL-27, and IL-35, all of which play important roles in autoimmunity. Thus far, the relationship between IL-12, IL-27, and IL-35 and the TAO has not been evaluated.
Seventy-five serum samples from patients with TAO were collected. Serum samples from 90 healthy controls (HC), 55 patients with Graves' disease (GD), 38 patients with uveitis (UV), 17 patients with Sjogren's syndrome (SS), and 65 patients with rheumatoid arthritis (RA) were collected as controls. The associations between IL-27, IL-35, IL-12, and other clinical parameters were analyzed.
Elevated serum levels of IL-27/IL-35 and decreased serum IL-12 levels were observed in TAO patients compared to those in HC (p < 0.001). For HC, we observed good diagnostic ability to predict TAO (area under the curve = 0.74, 0.78, and 0.78, for IL-27, IL-35, and IL-12, respectively). For other autoimmune diseases, IL-27, IL-35, and IL-12 had the ability to discriminate between UV, RA, and SS (area under the curve = 0.80, 0.83, and 0.85 for IL-27; 0.52, 0.69, and 0.67 for IL-35). The positive detection rates of IL-12 were significantly lower in the TAO group than in the UV and RA groups (p = 0.002, 0.01).
IL-12, IL-27, and IL-35 have the potential as biomarkers for TAO.
甲状腺相关眼病（TAO）是一种慢性自身免疫性疾病。白细胞介素-12（IL-12）家族包括IL-12、IL-23、IL-27和IL-35，它们在自身免疫中均发挥重要作用。迄今为止，IL-12、IL-27和IL-35与TAO之间的关系尚未得到评估。
收集75例TAO患者的血清样本。收集90例健康对照（HC）、55例格雷夫斯病（GD）患者、38例葡萄膜炎（UV）患者、17例干燥综合征（SS）患者和65例类风湿关节炎（RA）患者的血清样本作为对照。分析IL-27、IL-35、IL-12与其他临床参数之间的关联。
与HC相比，TAO患者血清中IL-27/IL-35水平升高，IL-12水平降低（p < 0.001）。对于HC，我们观察到其对TAO具有良好的诊断能力（IL-27、IL-35和IL-12的曲线下面积分别为0.74、0.78和0.78）。对于其他自身免疫性疾病，IL-27、IL-35和IL-12能够区分UV、RA和SS（IL-27的曲线下面积为0.80、0.83和0.85；IL-35的曲线下面积为0.52、0.69和0.67）。TAO组中IL-12的阳性检出率显著低于UV组和RA组（p = 0.002，0.01）。
IL-12、IL-27和IL-35有潜力作为TAO的生物标志物。
