Shen Jie, Li Zhangfang, Li Wenting, Ge Ying, Xie Min, Lv Meng, Fan Yanfei, Chen Zhi, Zhao Defu, Han Yajuan
Department of Endocrinology and Metabolism, The Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, China.
Department of Endocrinology and Metabolism, Guangzhou City People's Hospital, Huadu District, Guangzhou, China.
Dis Markers. 2015;2015:609593. doi: 10.1155/2015/609593. Epub 2015 May 18.
To determine serum cytokine profiles in Graves' disease (GD) patients with or without active and inactive thyroid associated ophthalmopathy (TAO), we recruited 65 subjects: 10 GD only (without TAO), 25 GD + active TAO, 20 GD + TAO, and 10 healthy controls. Liquid chip assay was used to measure serum Th1/Th2/Th17 cytokines including IFN-γ (interferon-gamma), TNF-α (tumor necrosis factor-alpha), IL-1α (interleukin-1 alpha), IL-1Ra (IL-1 receptor antagonist), IL-2, IL-4, IL-6, and IL-17 and two chemokines: RANTES (regulated upon activation, normal T cell expressed and secreted) and IP-10 (IFN-γ-induced protein 10). Serum levels of TSH (thyroid stimulating hormone) receptor autoantibodies (TRAb) were measured using an enzyme linked immunosorbent assay. Compared with healthy controls, TAO patients showed significantly elevated serum levels of IFN-γ, TNF-α, IL-1α, IL-4, IL-6, IL-17, and IP-10. Comparing active and inactive TAO, serum Th1 cytokines IFN-γ and TNF-α were elevated in active TAO, while serum Th2 cytokine IL-4 was elevated in inactive TAO. Serum Th17 cytokine IL-17 was elevated in GD but reduced in both active and inactive TAO. A positive correlation was found between TRAb and IFN-γ, TNF-α, IL-1α, IL-2, IL-4, and IL-6. Taken together, serum Th1/Th2/Th17 cytokines and chemokines reflect TAO disease activity and may be implicated in TAO pathogenesis.
为了确定伴有或不伴有活动期和静止期甲状腺相关眼病（TAO）的格雷夫斯病（GD）患者的血清细胞因子谱，我们招募了65名受试者：10名仅患有GD（无TAO）、25名GD + 活动期TAO、20名GD + TAO以及10名健康对照。采用液相芯片分析法检测血清Th1/Th2/Th17细胞因子，包括干扰素-γ（IFN-γ）、肿瘤坏死因子-α（TNF-α）、白细胞介素-1α（IL-1α）、白细胞介素-1受体拮抗剂（IL-1Ra）、白细胞介素-2、白细胞介素-4、白细胞介素-6和白细胞介素-17，以及两种趋化因子：活化正常T细胞表达和分泌的调节因子（RANTES）和干扰素-γ诱导蛋白10（IP-10）。采用酶联免疫吸附测定法检测血清促甲状腺激素（TSH）受体自身抗体（TRAb）水平。与健康对照相比，TAO患者血清中IFN-γ、TNF-α、IL-1α、IL-4、IL-6、IL-17和IP-10水平显著升高。比较活动期和静止期TAO，活动期TAO患者血清Th1细胞因子IFN-γ和TNF-α升高，而静止期TAO患者血清Th2细胞因子IL-4升高。血清Th17细胞因子IL-17在GD患者中升高，但在活动期和静止期TAO患者中均降低。发现TRAb与IFN-γ、TNF-α、IL-1α、IL-2、IL-4和IL-6之间呈正相关。综上所述，血清Th1/Th2/Th17细胞因子和趋化因子反映了TAO的疾病活动，可能参与了TAO的发病机制。
