Cong Shanshan, Wang Chao, Wei Tianli, Xie Zhiping, Huang Yiman, Tan Jingjing, Chen Aijun, Ma Fenlian, Zheng Lishu
NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China; Department of Toxicology, School of Public Health, China Medical University, Shenyang, China.
NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
Infect Genet Evol. 2022 Dec;106:105386. doi: 10.1016/j.meegid.2022.105386. Epub 2022 Nov 11.
This study aims to described the epidemiology and genotypic diversity of Human metapneumovirus (HMPV) and the impact of SARS-CoV-2 on the prevalence of HMPV in hospitalized children with Acute respiratory tract infections (ARTIs) in Beijing, China.
From April 2018 to March 2019 and from September 2020 to August 2021, nasopharyngeal aspirates (NPAs) from hospitalized children with ARTIs in Beijing were collected and subjected to real-time polymerase chain reaction tests for HMPV. Then genotyping, detection of 15 common respiratory viruses and clinical characteristics were analyzed on HMPV positive samples.
7.9% (124/1572) enrolled pediatric patients were identified as having HMPV infection, and the majority of children under the age of 5 (78.2%, 92/124), From April 2018 to March 2019. The detection rate of HMPV in spring and winter is significantly higher than that in summer and autumn. The co-infection rate were 37.1% (46/124), the most common co-infected virus were parainfluenza virus type 3 (HPIV-3). The main diagnosis of HMPV infection was pneumonia (92.7%,115/124), most patient have cough and fever. Of 78 HMPV-positive specimens, A2b (82.1%,64/78) were the main epidemic subtypes. Hospitalized children with HMPV genotype A infection had a higher viral load compared to genotype B. During the COVID-19 outbreak, Among 232 samples, only 4 cases were HMPV-positive. After statistical test, the detection rate of HMPV during the COVID-19 pandemic has decreased significantly compared with that before the epidemic (p = 0.001).
HMPV is an important cause of ARTIs in children under 5 years old. The epidemic peak is generally in winter and spring, and the A2b subtype is the most common. However, under the prevention and control of the COVID-19 pandemic, the HMPV infection of hospitalized children with ARTIs has decreased significantly.
本研究旨在描述人偏肺病毒（HMPV）的流行病学和基因多样性，以及新型冠状病毒（SARS-CoV-2）对中国北京住院的急性呼吸道感染（ARTIs）儿童中HMPV流行率的影响。
在2018年4月至2019年3月以及2020年9月至2021年8月期间，收集北京住院的ARTIs儿童的鼻咽抽吸物（NPAs），并对其进行HMPV的实时聚合酶链反应检测。然后对HMPV阳性样本进行基因分型、15种常见呼吸道病毒的检测以及临床特征分析。
1572名登记的儿科患者中有7.9%（124/1572）被确定感染了HMPV，且大多数为5岁以下儿童（78.2%，92/124）。在2018年4月至2019年3月期间，HMPV在春季和冬季的检出率显著高于夏季和秋季。合并感染率为37.1%（46/124），最常见的合并感染病毒是3型副流感病毒（HPIV-3）。HMPV感染的主要诊断为肺炎（92.7%，115/124），大多数患者有咳嗽和发热症状。在78份HMPV阳性标本中，A2b型（82.1%，64/78）是主要的流行亚型。与B型基因型相比，感染HMPV A型基因型的住院儿童病毒载量更高。在新冠疫情期间，在232份样本中，仅4例HMPV呈阳性。经统计学检验，新冠疫情期间HMPV的检出率与疫情前相比显著下降（p = 0.001）。
HMPV是5岁以下儿童ARTIs的重要病因。流行高峰一般在冬季和春季，且A2b亚型最为常见。然而，在新冠疫情防控下，住院的ARTIs儿童的HMPV感染显著下降。
