Jiangsu Key Laboratory of Chiral Pharmaceuticals Biosynthesis, Taizhou University, Taizhou, 225300, P. R. China.
Center for Novel Drug Development of Taizhou, Taizhou, 225300, P. R. China.
Chem Biodivers. 2022 Dec;19(12):e202200993. doi: 10.1002/cbdv.202200993. Epub 2022 Dec 3.
A new iridoid glycoside, named 6'-O-trans-feruloyl-8-epiloganic acid, together with fifteen known compounds were isolated from the twigs and leaves of Callicarpa nudiflora, a traditional Chinese medicine to treat inflammatory-related diseases. Their structures were identified by comprehensive spectroscopic analysis and comparison with reported data. Bioassay results revealed that twelve of the isolates could obviously inhibit nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW 264.7 cell lines with IC values from 0.64 to 38.72 μM. Among them, compounds 1 (3.27 μM), 6 (5.23 μM), 13 (1.56 μM) and 14 (0.64 μM) exhibited significantly higher activities than that of the positive control (27.13 μM). Additionally, it was supposed that the presence of the carboxy group at the C-4 position of iridoid glycosides and glycosylation at C-3 position of flavonoids might impact their inhibitory activities against NO production.
从传统中药治疗炎症相关疾病的黑老虎（Callicarpa nudiflora）的嫩枝和叶片中分离得到一个新的环烯醚萜糖苷，命名为 6'-O-反式-阿魏酰-8-表长春苷酸，以及十五个已知化合物。通过综合光谱分析和与报道数据的比较，确定了它们的结构。生物测定结果表明，其中 12 种分离物对脂多糖（LPS）诱导的 RAW 264.7 细胞系中一氧化氮（NO）的产生具有明显的抑制作用，IC 值范围为 0.64 至 38.72 μM。其中，化合物 1（3.27 μM）、6（5.23 μM）、13（1.56 μM）和 14（0.64 μM）的活性明显高于阳性对照（27.13 μM）。此外，推测环烯醚萜糖苷 C-4 位的羧基和黄酮 C-3 位的糖苷化可能影响其对 NO 产生的抑制活性。
