Engineering Technology Research Center of Jiangxi Universities and Colleges for Selenium Agriculture, College of Life Science and Environmental Resources, Yichun University, Yichun, 336000, China.
School of Geography, Nanjing Normal University, Nanjing, 210023, China.
Microb Ecol. 2023 Aug;86(2):1132-1144. doi: 10.1007/s00248-022-02139-w. Epub 2022 Nov 14.
Reductive soil disinfestation (RSD) incorporated with sole plant residues or liquid-readily decomposable compounds is an effective management strategy to improve soil health. However, the synthetic effects of RSD incorporated with liquid-readily decomposable compounds and solid plant residues on soil ecosystem services remain unclear. Field experiments were carried out to investigate the effects of untreated soil (CK), RSD incorporated with sawdust (SA), molasses (MO), and their combinations (SA + MO) on the bacterial community and functional composition. The results showed that RSD treatments significantly altered soil bacterial community structure compared to CK treatment. The bacterial community structure and composition in MO and SA + MO treatments were clustered compared to SA treatment. This was mainly attributed to the readily decomposable carbon sources in molasses having a stronger driving force to reshape the soil microbial community during the RSD process. Furthermore, the functional compositions, such as the disinfestation efficiency of F. oxysporum (96.4 - 99.1%), abundances of nitrogen functional genes, soil metabolic activity, and functional diversity, were significantly increased in all of the RSD treatments. The highest disinfestation efficiency and abundances of denitrification (nirS and nrfA) and nitrogen fixation (nifH) genes were observed in SA + MO treatment. Specifically, SA + MO treatment enriched more abundant beneficial genera, e.g., Oxobacter, Paenibacillus, Cohnella, Rummeliibacillus, and Streptomyces, which were significantly and positively linked to disinfestation efficiency, soil metabolic activity, and denitrification processes. Our results indicated that combining RSD practices with liquid-readily decomposable compounds and solid plant residues could effectively improve soil microbial community and functional composition.
还原土消毒（RSD）与单一植物残体或液体易分解化合物结合使用是改善土壤健康的有效管理策略。然而，RSD 与液体易分解化合物和固体植物残体结合使用对土壤生态系统服务的综合影响仍不清楚。进行了田间试验，以研究未处理土壤（CK）、木屑（SA）、糖蜜（MO）和它们的组合（SA+MO）对细菌群落和功能组成的影响。结果表明，与 CK 处理相比，RSD 处理显著改变了土壤细菌群落结构。与 SA 处理相比，MO 和 SA+MO 处理中的细菌群落结构和组成聚类。这主要归因于糖蜜中易分解的碳源在 RSD 过程中具有更强的驱动力来重塑土壤微生物群落。此外，消毒效率（F. oxysporum 为 96.4-99.1%）、氮功能基因丰度、土壤代谢活性和功能多样性等功能组成在所有 RSD 处理中均显著增加。在 SA+MO 处理中观察到最高的消毒效率和脱氮（nirS 和 nrfA）和固氮（nifH）基因丰度。具体而言，SA+MO 处理富集了更丰富的有益属，例如 Oxobacter、Paenibacillus、Cohnella、Rummeliibacillus 和 Streptomyces，它们与消毒效率、土壤代谢活性和脱氮过程显著正相关。我们的结果表明，将 RSD 实践与液体易分解化合物和固体植物残体结合使用可以有效改善土壤微生物群落和功能组成。
