Facilitating effects of the reductive soil disinfestation process combined with sp. amendment on soil health and physiological properties of .

Reductive soil disinfestation (RSD) is an anaerobic and facultative anaerobic microbial-mediated soil management process. The extent of improvement of diseased soil properties by RSD relative to comparable healthy soil is, however, not well characterized. Importantly, how to promote the colonization efficiency of these facultative anaerobic functional species to ensure soil and plant health remain unknown. Here, wilt-diseased soil of grown under a plastic-shed field (PS-CK) was used to conduct molasses-RSD (MO-RSD) along with sp. (a model of facultative anaerobic species) (MO-RSD) treatment, and the soil from a nearby open-air paddy field was considered comparable healthy soil (OA-CK). Both RSD treatments significantly improved the properties of PS-CK soil, and the extent of improvement of soil pH, reduction efficiency (98.36%~99.56%), and microbial community and functional composition were higher than that achieved for OA-CK soil, which indicated that RSD-regulated most soil properties outperformed those of the comparable healthy soil. The disease incidence and ascorbic acid content of in MO-RSD- and MO-RSD-treated soils were considerably decreased, while the weight and soluble protein contents were correspondingly increased, as compared to those of in PS-CK soil. Specifically, the changes in these physiological properties of in MO-RSD soil performed well than that in MO-RSD soil. The relative abundances of , , , , , and enriched in both RSD-treated soils were positively correlated with and negatively correlated with population and disease incidence ( < 0.05). Notably, the relative abundances of these potential probiotics were considerably higher in MO-RSD-treated soil than in MO-RSD alone-treated soil. These results show that the RSD process with inoculation of sp. could promote the colonization of this species and simultaneously stimulate the proliferation of other probiotic consortia to further enhance soil health and plant disease resistance.