Department of Surgery, The Ohio State University Wexner Medical Center, Columbus, OH, USA.
Department of Physiology and Cell Biology, University of Nevada, Reno School of Medicine, Reno, NV, USA.
Methods Mol Biol. 2023;2597:1-9. doi: 10.1007/978-1-0716-2835-5_1.
Hindlimb suspension is a well-established rodent model of disuse-induced atrophy and is commonly used to simulate the effects of bed rest and space flight on humans. Over the decades, this method has undergone many changes to reduce the stress response on the animals and improve the reliability of the data. Here, we detail our method of performing hindlimb suspension in mice that minimizes stress, maximizes the replicability of the data, and uses space efficiently.
下肢悬吊是一种成熟的废用性萎缩啮齿动物模型，常用于模拟卧床和太空飞行对人类的影响。几十年来，这种方法经历了许多变化，以减少动物的应激反应，提高数据的可靠性。在这里，我们详细介绍了一种在小鼠中进行下肢悬吊的方法，该方法最大限度地减少了应激，最大限度地提高了数据的可重复性，并有效地利用了空间。
