糖胺聚糖的分离与组成分析。
Isolation and Compositional Analysis of Glycosaminoglycans.
机构信息
University of Georgia, Complex Carbohydrate Research Center, Athens, GA, USA.
出版信息
Methods Mol Biol. 2023;2597:177-186. doi: 10.1007/978-1-0716-2835-5_14.
Abstract
The compositional and structural analysis of GAGs is challenging due to their heterogenous structures. Strong anion exchange (SAX) HPLC can aid in the compositional analysis of GAGs and can separate complex mixtures based on charge and degree of sulfation. Herein we describe the digestion and release of GAGs from tissue, and the compositional analysis using SAX-HPLC.
摘要
由于糖胺聚糖 (GAGs) 的结构具有异质性，因此其组成和结构分析具有一定挑战性。强阴离子交换 (SAX) HPLC 可辅助 GAG 组成分析，并可根据电荷和硫酸化程度分离复杂混合物。本文描述了从组织中消化和释放 GAGs 以及使用 SAX-HPLC 进行组成分析的方法。
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