Rice K G, Kim Y S, Grant A C, Merchant Z M, Linhardt R J
Anal Biochem. 1985 Nov 1;150(2):325-31. doi: 10.1016/0003-2697(85)90518-4.
Heparin has been enzymatically depolymerized with heparinase (heparin lyase (EC 4.2.2.7)) and then separated into di-, tetra-, hexa-, octa-, and decasaccharide mixtures by low-pressure gel-permeation chromatography (GPC). These sized mixtures were resolved by strong anion-exchange (SAX) HPLC into multiple components. The fractions from the SAX-HPLC were collected and characterized for size by GPC-HPLC and sulfate content by ion chromatography. This study provides detailed methodology for the separation of larger and more highly sulfated oligosaccharides than previously reported. It describes the first use of ion chromatography for the accurate determination of the sulfate content of heparin oligosaccharides, a method which can also be applied to heparin and other glycosaminoglycans.
肝素已用肝素酶（肝素裂解酶（EC 4.2.2.7））进行酶促解聚，然后通过低压凝胶渗透色谱法（GPC）分离成二糖、四糖、六糖、八糖和十糖混合物。这些按大小分级的混合物通过强阴离子交换（SAX）HPLC分离为多个组分。收集SAX-HPLC的馏分，并通过GPC-HPLC对其大小进行表征，通过离子色谱法对其硫酸盐含量进行表征。本研究提供了比以前报道的更详细的分离更大且硫酸化程度更高的寡糖的方法。它描述了首次使用离子色谱法准确测定肝素寡糖的硫酸盐含量，该方法也可应用于肝素和其他糖胺聚糖。
