Blood Cell Development and Function Program, Fox Chase Cancer Center, Philadelphia, PA, USA.
Methods Mol Biol. 2023;2580:355-377. doi: 10.1007/978-1-0716-2740-2_22.
While the zebrafish has for some time been regarded as a powerful model organism with which to study early events in hematopoiesis, recent evidence suggests that it also ideal for unraveling the molecular requirements for T cell development in the thymus. Like mammals, zebrafish possess an adaptive immune system, comprising B lymphocytes as well as both the γδ and αβ lineages of T cells, which develop in the thymus. Moreover, the molecular processes underlying T cell development in zebrafish appear to be remarkably conserved. Thus, findings in the zebrafish model will be of high relevance to the equivalent processes in mammals. Finally, molecular processes can be interrogated in zebrafish far more rapidly than is possible in mammals because the zebrafish possesses many unique advantages. Here, we describe these unique attributes and the methods by which they can be exploited to investigate the role of novel genes in T cell development.
虽然斑马鱼在一段时间以来一直被认为是一种强大的模式生物，可用于研究造血过程中的早期事件，但最近的证据表明，它也非常适合揭示胸腺中 T 细胞发育的分子要求。与哺乳动物一样，斑马鱼拥有适应性免疫系统，包括 B 淋巴细胞以及 T 细胞的γδ和αβ谱系，这些细胞在胸腺中发育。此外，斑马鱼中 T 细胞发育的分子过程似乎非常保守。因此，斑马鱼模型中的发现将与哺乳动物中相应的过程高度相关。最后，由于斑马鱼具有许多独特的优势，因此可以比在哺乳动物中更快地研究分子过程。在这里，我们描述了这些独特的属性以及利用它们来研究新型基因在 T 细胞发育中的作用的方法。
