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超分辨率重扫二次谐波产生显微镜。

Super-resolution re-scan second harmonic generation microscopy.

机构信息

Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 060042 Bucharest, Romania.

Pathology Department, Emergency Military Hospital, 010825 Bucharest, Romania.

出版信息

Proc Natl Acad Sci U S A. 2022 Nov 22;119(47):e2214662119. doi: 10.1073/pnas.2214662119. Epub 2022 Nov 14.

DOI:10.1073/pnas.2214662119
PMID:36375085
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9704717/
Abstract

Second harmonic generation microscopy (SHG) is generally acknowledged as a powerful tool for the label-free three-dimensional visualization of tissues and advanced materials, with one of its most popular applications being collagen imaging. Despite the great need, progress in super-resolved SHG imaging lags behind the developments reported over the past years in fluorescence-based optical nanoscopy. In this work, we demonstrate super-resolved re-scan SHG, qualitatively and quantitatively showing on collagenous tissues the available resolution advantage over the diffraction limit. We introduce as well super-resolved re-scan two-photon excited fluorescence microscopy, an imaging modality not explored to date.

摘要

二次谐波产生显微镜 (SHG) 通常被认为是一种强大的工具，可用于无标记的组织和先进材料的三维可视化，其最受欢迎的应用之一是胶原蛋白成像。尽管有巨大的需求，但在超分辨 SHG 成像方面的进展落后于过去几年中基于荧光的光学纳米技术的发展。在这项工作中，我们展示了超分辨再扫描 SHG，定性和定量地显示在胶原组织上相对于衍射极限的可用分辨率优势。我们还介绍了超分辨再扫描双光子激发荧光显微镜，这是一种迄今为止尚未探索的成像模式。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c0e/9704717/9db07f0d8379/pnas.2214662119fig03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c0e/9704717/907623812914/pnas.2214662119fig01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c0e/9704717/194498183dca/pnas.2214662119fig02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c0e/9704717/9db07f0d8379/pnas.2214662119fig03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c0e/9704717/907623812914/pnas.2214662119fig01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c0e/9704717/194498183dca/pnas.2214662119fig02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c0e/9704717/9db07f0d8379/pnas.2214662119fig03.jpg

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