3rd Institute of Physics - Biophysics, University of Göttingen, Göttingen, Germany.
Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB), Göttingen, Germany.
Nat Methods. 2017 Nov;14(11):1087-1089. doi: 10.1038/nmeth.4467. Epub 2017 Oct 16.
Image scanning microscopy (ISM) doubles the resolution of a conventional confocal microscope for super-resolution imaging. Here, we describe an all-optical ISM design based on rescanning microscopy for two-photon-excited fluorescence and second-harmonic generation that allows straightforward implementation into existing microscopes. The design offers improved sensitivity and high frame rates relative to those of existing systems. We demonstrate its utility using fixed and living specimens as well as collagen hydrogels.
图像扫描显微镜（ISM）将传统共聚焦显微镜的分辨率提高了一倍，可用于超分辨率成像。本文描述了一种基于重扫描显微镜的全光学 ISM 设计，用于双光子激发荧光和二次谐波产生，可直接集成到现有显微镜中。与现有系统相比，该设计具有更高的灵敏度和更高的帧率。我们使用固定和活的样本以及胶原水凝胶证明了其效用。
