Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, People's Republic of China.
Nano Lett. 2023 Sep 13;23(17):7975-7982. doi: 10.1021/acs.nanolett.3c01903. Epub 2023 Aug 29.
Second-harmonic generation (SHG) is a noninvasive imaging technique that enables the exploration of physiological structures without the use of an exogenous label. However, traditional SHG imaging is limited by optical diffraction, which restricts the spatial resolution. To break this limitation, we developed a novel approach called multifocal structured illumination microscopy-SHG (MSIM-SHG). By combination of SHG with MSIM, SHG-based super-resolution imaging of material molecules can be achieved, and this SHG super-resolution imaging has a wide range of applications for biological tissues and cells. MSIM-SHG achieved a lateral full width at half-maximum (fwhm) of 147 ± 13 nm and an axial fwhm of 493 ± 47 nm by imaging zinc oxide (ZnO) particles. Furthermore, MSIM-SHG was utilized to quantify collagen fiber alignment in various tissues such as the ovary, muscle, heart, kidney, and cartilage, demonstrating its feasibility for identifying collagen characteristics. MSIM-SHG has potential as a powerful tool for clinical diagnosis and biological research.
二次谐波产生（SHG）是一种非侵入性的成像技术，可在不使用外源性标记的情况下探索生理结构。然而，传统的 SHG 成像受到光衍射的限制，这限制了空间分辨率。为了打破这一限制，我们开发了一种称为多焦点结构光照明显微镜-SHG（MSIM-SHG）的新方法。通过将 SHG 与 MSIM 结合，可以实现基于 SHG 的材料分子超分辨率成像，这种 SHG 超分辨率成像在生物组织和细胞中有广泛的应用。通过对氧化锌（ZnO）颗粒进行成像，MSIM-SHG 实现了 147±13nm 的横向半高全宽（fwhm）和 493±47nm 的轴向 fwhm。此外，MSIM-SHG 用于定量分析卵巢、肌肉、心脏、肾脏和软骨等各种组织中的胶原纤维排列，证明了其识别胶原特征的可行性。MSIM-SHG 有望成为临床诊断和生物研究的有力工具。
