Hou Xianliang, Wang Ying-Lan, Shi Wei, Hu Wenlong, Zeng Zhipeng, Liu Jiayi, Li Lian, Cai Wanxia, Tang Donge, Dai Yong
Department of Clinical Medical Research Center, Guangdong Provincial Engineering Research Center of Autoimmune Disease Precision Medicine, The Second Clinical Medical College, Jinan University (Shenzhen People's Hospital), Shenzhen 518020, China; The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong 510632, China.
Shenzhen Far East Women & Children Hospital, Shenzhen 518000, Guangdong, China.
Mol Immunol. 2022 Dec;152:207-214. doi: 10.1016/j.molimm.2022.10.013. Epub 2022 Nov 11.
Nowadays, umbilical cord blood (UCB) cells has been increasingly replacing fetal and adult-derived cells in adoptive cell therapy. However, gene expression and chromatin accessibility in umbilical cord blood cells has not yet been fully elucidated. In this study, we used an integration of scRNA-seq with the scATAC-seq technology to perform unbiased analysis of UCBCs over developmental time from 31 gestational week (GW) to 37 GW in humans. We identified several distinct cell types (erythroid cell, T cell, B cell, erythroid precursor cells, NK cell, and endothelial progenitor cell) and subpopulations (6 different clusters of erythroid cells) in UCB cells. In addition, we also identified a series of differentially expressed genes and chromatin accessibility in each cell type between different gestational weeks. Interestingly, the gene expression pattern of umbilical cord blood cells from normal fetuses of similar gestational weeks were more consistent. In conclusion, our analysis presents a better understanding of the chromatin landscape and regulatory networks in UCB cells.
如今，在过继性细胞疗法中，脐带血细胞越来越多地取代了胎儿和成人来源的细胞。然而，脐带血细胞中的基因表达和染色质可及性尚未得到充分阐明。在本研究中，我们整合了scRNA-seq和scATAC-seq技术，对人类从妊娠31周（GW）到37周的发育过程中的脐带血细胞进行了无偏分析。我们在脐带血细胞中鉴定出了几种不同的细胞类型（红细胞、T细胞、B细胞、红细胞前体细胞、NK细胞和内皮祖细胞）和亚群（6个不同的红细胞簇）。此外，我们还鉴定了不同孕周之间每种细胞类型中一系列差异表达的基因和染色质可及性。有趣的是，孕周相似的正常胎儿的脐带血细胞的基因表达模式更为一致。总之，我们的分析更好地揭示了脐带血细胞中的染色质景观和调控网络。
