使用 scRNA-Seq 和 scATAC-Seq 进行视网膜基因表达和染色质可及性的多重分析。
Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility using scRNA-Seq and scATAC-Seq.
机构信息
Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine.
Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine; Department of Psychiatry and Behavioral Science, The Johns Hopkins Hospital; Department of Ophthalmology, The Johns Hopkins Hospital; Department of Neurology, The Johns Hopkins Hospital; Institute for Cell Engineering, Johns Hopkins University School of Medicine; Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine;
出版信息
J Vis Exp. 2021 Mar 12(169). doi: 10.3791/62239.
Powerful next generation sequencing techniques offer robust and comprehensive analysis to investigate how retinal gene regulatory networks function during development and in disease states. Single-cell RNA sequencing allows us to comprehensively profile gene expression changes observed in retinal development and disease at a cellular level, while single-cell ATAC-Seq allows analysis of chromatin accessibility and transcription factor binding to be profiled at similar resolution. Here the use of these techniques in the developing retina is described, and MULTI-Seq is demonstrated, where individual samples are labeled with a modified oligonucleotide-lipid complex, enabling researchers to both increase the scope of individual experiments and substantially reduce costs.
强大的新一代测序技术提供了强大而全面的分析，可研究视网膜基因调控网络在发育过程中和疾病状态下的功能。单细胞 RNA 测序使我们能够在细胞水平上全面分析视网膜发育和疾病中观察到的基因表达变化，而单细胞 ATAC-Seq 则可以分析染色质可及性和转录因子结合，分辨率相似。本文描述了这些技术在发育中的视网膜中的应用，并展示了 MULTI-Seq，其中每个样本都用修饰的寡核苷酸-脂质复合物进行标记，使研究人员能够扩大单个实验的范围，并大大降低成本。
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