Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta T6G 2G6, Canada.
Anton Paar Canada Inc., 4920 Place Olivia, Montreal, Quebec, H4R 2Z8, Canada.
Biomater Sci. 2022 Dec 20;11(1):128-139. doi: 10.1039/d2bm01055k.
Infections caused by biofouling have become a serious concern in the health care sector. Multifunctional coatings with antifouling and antibacterial properties are widely used to combat these biofouling related infections. However, in practice macro or micro scratches or damages can happen to the coating, which can act as an active site for microbial deposition and destroy the antifouling or antibacterial functionality of the coating. Considering this fact, we have developed an excellent biocompatible and multifunctional coating with antifouling, antibacterial and self-healing properties. In this study, prebiotic chemistry inspired self-polymerization of aminomalononitrile (AMN) was used as a primary coating layer, which acted as a primer to graft vitamin B5 analogous methacrylamide polymer poly(B5AMA) and zwitterionic compound 2-methacryloyloxyethyl phosphorylcholine (MPC) containing polymer poly (MPC--B5AMA) by forming strong hydrogen bonds. B5AMA having multiple polar groups in the structure acted as an intrinsic self-healing material and showed an excellent antifouling property against protein and bacteria, maintaining a good hydration layer similar to the MPC containing polymer. To impart the antibacterial property to the coating, silver nanoparticles have also been incorporated, which showed more than 90% killing efficiency against both Gram-positive () and Gram-negative () bacteria with significant reduction of their adhesion on the surface. Incorporation of self-healing property into the fouling repelling and antibacterial coating can significantly extend the durability of the multifunctional coating, making it promising for biomedical applications.
生物污垢引起的感染已成为医疗保健领域的严重问题。具有抗生物污垢和抗菌性能的多功能涂层被广泛用于对抗这些与生物污垢相关的感染。然而，在实际中，涂层可能会出现宏观或微观的划痕或损坏，这些划痕或损坏可以作为微生物沉积的活性部位，并破坏涂层的抗生物污垢或抗菌功能。考虑到这一事实，我们开发了一种具有良好生物相容性和多功能的抗生物污垢、抗菌和自修复性能的涂层。在这项研究中，受前生物化学启发的氨甲腈（AMN）自聚合被用作初级涂层，作为接枝维生素 B5 类似物甲基丙烯酰胺聚合物聚（B5AMA）和两性离子化合物 2-甲基丙烯酰氧基乙基磷酸胆碱（MPC）的接枝剂聚合物聚（MPC-B5AMA）通过形成强氢键。B5AMA 结构中含有多个极性基团，充当内在自修复材料，并表现出优异的抗蛋白质和细菌污染性能，保持类似于含 MPC 的聚合物的良好水合层。为了赋予涂层抗菌性能，还掺入了银纳米粒子，它们对革兰氏阳性菌（）和革兰氏阴性菌（）的杀灭效率均超过 90%，并显著减少了它们在表面的粘附。将自修复性能纳入防污和抗菌涂层中，可以显著延长多功能涂层的耐久性，使其在生物医学应用中具有广阔的前景。
