Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland.
Methods Mol Biol. 2023;2596:265-289. doi: 10.1007/978-1-0716-2831-7_19.
The gradual loss of skeletal muscle mass during aging and associated decline in contractile strength can result in reduced fitness, frailty, and loss of independence. In order to better understand the molecular and cellular mechanisms that underlie sarcopenia of old age and the frailty syndrome, as well as identify novel therapeutic targets to treat age-related fiber wasting, it is crucial to develop a comprehensive biomarker signature of muscle aging. Fluorescence two-dimensional gel electrophoresis (2D-DIGE) in combination with sensitive mass spectrometry presents an ideal bioanalytical tool for biomarker discovery in biogerontology. This chapter outlines the application of the 2D-DIGE method for the comparative analysis of human biopsy specimens from middle-aged versus senescent individuals using a two-CyDye-based method.
随着年龄的增长，骨骼肌逐渐减少，收缩力下降，会导致身体机能下降、虚弱和丧失独立性。为了更好地理解导致老年肌肉减少症和衰弱综合征的分子和细胞机制，以及确定治疗与年龄相关的纤维消耗的新的治疗靶点，开发一种全面的肌肉老化生物标志物特征至关重要。荧光二维凝胶电泳（2D-DIGE）结合灵敏的质谱分析为生物衰老学中的生物标志物发现提供了一种理想的生物分析工具。本章概述了使用基于两种 CyDye 的方法，应用 2D-DIGE 方法对中年和老年个体的活检样本进行比较分析的方法。
