Sultana Sajeda, Pervin Munmun, Sultana Nazneen, Siddique Mahbubul Pratik, Islam Md Rafiqul, Khan Mohammad Abu Hadi Noor Ali
Department of Pathology, Faculty of Animal Science and Veterinary Medicine, Sher e Bangla Agricultural University, Dhaka, Bangladesh.
Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
J Adv Vet Anim Res. 2022 Sep 30;9(3):463-470. doi: 10.5455/javar.2022.i615. eCollection 2022 Sep.
Peste des petits ruminants (PPR) virus is the main infectious cause of goat mortality in Bangladesh, and co-infection may make diseases more severe. This study aimed to detect PPR and co-infecting diseases in goats.
One hundred goats suspected to be infected with the PPR virus were collected from various areas of Mymensingh district, Bangladesh. A systemic post-mortem examination was carried out on PPR-suspected goats. Lungs, spleen, and lymph nodes (pre-scapular) were used for ribonucleic acid extraction, whereas lungs and mesenteric lymph nodes were used for deoxyribonucleic acid extraction. Seven-pair primer sets were used for molecular detection of pathogens specific for PPR, goat pox, contagious ecthyma (Orf), foot and mouth disease (FMD) virus, sp., and sp. Reverse transcriptase-polymerase chain reaction (RT-PCR) or polymerase chain reaction (PCR) were used to find the exact cause.
Out of 100 PPR-suspected goats examined, 55 goats were confirmed as PPR-detected by RT-PCR. Among the 55 PPR-positive goats, 2 were co-infected with goat pox, 2 with tuberculosis, 10 with sp. infection, and 6 with FMD as detected by PCR and RT-PCR. Moreover, 12 goats were co-infected with PPRV and fascioliasis.
About 58% of PPR virus-infected goats were co-infected with other organisms. There is a need to design technology to detect the state of co-infectivity at its early onset and future preventive and therapeutic strategies for co-infecting diseases. This is the first study in Bangladesh to describe co-infecting diseases of goats along with PPR.
小反刍兽疫（PPR）病毒是孟加拉国山羊死亡的主要传染性病因，合并感染可能使疾病更加严重。本研究旨在检测山羊中的PPR及合并感染的疾病。
从孟加拉国迈门辛区的不同地区收集了100只疑似感染PPR病毒的山羊。对疑似感染PPR的山羊进行了系统的尸检。取肺、脾和（肩胛前）淋巴结用于核糖核酸提取，而取肺和肠系膜淋巴结用于脱氧核糖核酸提取。使用七对引物组对PPR、山羊痘、传染性脓疱（羊口疮）、口蹄疫（FMD）病毒、 种和 种病原体进行分子检测。采用逆转录聚合酶链反应（RT-PCR）或聚合酶链反应（PCR）来找出确切病因。
在检查的100只疑似感染PPR的山羊中，有55只通过RT-PCR确诊感染PPR。在这55只PPR阳性山羊中，通过PCR和RT-PCR检测发现，有2只合并感染山羊痘，2只合并感染结核病，10只合并感染 种感染，6只合并感染FMD。此外，有12只山羊合并感染PPRV和肝片吸虫病。
约58%感染PPR病毒的山羊合并感染了其他生物体。有必要设计技术以在早期检测合并感染状态，并制定针对合并感染疾病的未来预防和治疗策略。这是孟加拉国第一项描述山羊PPR合并感染疾病的研究。
