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从耐旱小麦种子微生物组中进行有益微生物的分析、分离和特性分析。

Profiling, isolation and characterisation of beneficial microbes from the seed microbiomes of drought tolerant wheat.

机构信息

Agriculture Victoria Research, Department of Jobs, Precincts and Regions, AgriBio, Centre for AgriBioscience, Bundoora, VIC, Australia.

Agriculture Victoria Research, Department of Jobs, Precincts and Regions, AgriBio, Centre for AgriBioscience, 5 Ring Road, Bundoora, VIC, 3083, Australia.

出版信息

Sci Rep. 2021 Jun 7;11(1):11916. doi: 10.1038/s41598-021-91351-8.

DOI:10.1038/s41598-021-91351-8
PMID:34099781
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8184954/
Abstract

Climate change is predicted to increase the incidence and severity of drought conditions, posing a significant challenge for agriculture globally. Plant microbiomes have been demonstrated to aid crop species in the mitigation of drought stress. The study investigated the differences between the seed microbiomes of drought tolerant and drought susceptible wheat lines. Furthermore, it highlighted and quantified the degree of drought tolerance conferred by specific microbes isolated from drought tolerant wheat seed microbiomes. Metagenomic and culture-based methods were used to profile and characterise the seed microbiome composition of drought tolerant and drought susceptible wheat lines under rainfed and drought conditions. Isolates from certain genera were enriched by drought tolerant wheat lines when placed under drought stress. Wheat inoculated with isolates from these targeted genera, such as Curtobacterium flaccumfaciens (Cf D3-25) and Arthrobacter sp. (Ar sp. D4-14) demonstrated the ability to promote growth under drought conditions. This study indicates seed microbiomes from genetically distinct wheat lines enrich for beneficial bacteria in ways that are both line-specific and responsive to environmental stress. As such, seed from stress-phenotyped lines represent an invaluable resource for the identification of beneficial microbes with plant growth promoting activity that could improve commercial crop production.

摘要

气候变化预计会增加干旱条件的发生和严重程度，这对全球农业构成了重大挑战。植物微生物组已被证明有助于作物品种减轻干旱胁迫。本研究调查了耐旱性和耐旱性小麦品系种子微生物组之间的差异。此外，它还强调并量化了从耐旱性小麦种子微生物组中分离出的特定微生物赋予的耐旱性程度。本研究采用宏基因组学和基于培养的方法，在雨养和干旱条件下对耐旱性和耐旱性小麦品系的种子微生物组组成进行了分析和特征描述。在干旱胁迫下，某些属的分离物在耐旱性小麦品系中得到了富集。用这些目标属的分离物接种小麦，例如 Curtobacterium flaccumfaciens（Cf D3-25）和 Arthrobacter sp.（Ar sp. D4-14），证明了在干旱条件下促进生长的能力。本研究表明，遗传上不同的小麦品系的种子微生物组以特定于品系且对环境胁迫有反应的方式富集有益细菌。因此，具有胁迫表型的品系的种子代表了一种宝贵的资源，可以鉴定具有植物生长促进活性的有益微生物，从而提高商业作物的产量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae3f/8184954/bf0df5ea4e09/41598_2021_91351_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae3f/8184954/b0679418c84e/41598_2021_91351_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae3f/8184954/61bb7bee2d71/41598_2021_91351_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae3f/8184954/85be30f40641/41598_2021_91351_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae3f/8184954/23517f4fb58b/41598_2021_91351_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae3f/8184954/94445fa89f11/41598_2021_91351_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae3f/8184954/bf0df5ea4e09/41598_2021_91351_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae3f/8184954/b0679418c84e/41598_2021_91351_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae3f/8184954/61bb7bee2d71/41598_2021_91351_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae3f/8184954/85be30f40641/41598_2021_91351_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae3f/8184954/23517f4fb58b/41598_2021_91351_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae3f/8184954/94445fa89f11/41598_2021_91351_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae3f/8184954/bf0df5ea4e09/41598_2021_91351_Fig6_HTML.jpg

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