Joshi Kuldeep, Adhikari Priyanka, Bhatt Indra D, Pande Veena
Centre for Biodiversity Conservation and Management, G. B. Pant, National Institute of Himalayan Environment, Kosi- Katarmal, Almora, Uttarakhand 263 643 India.
Department of Biotechnology, Kumaun University, Bhimtal Campus, Bhimtal, Nainital, Uttarakhand 263 136 India.
Physiol Mol Biol Plants. 2022 Sep;28(9):1785-1798. doi: 10.1007/s12298-022-01242-z. Epub 2022 Oct 28.
is one of the ethno therapeutically important Himalayan plants used to cure various diseases. However, a systematic investigation of the effect of altitude on phytochemical, antioxidant, and antimicrobial activity has not been reported so far. The present study assessed the variation in the bioactive compounds, antioxidant and antimicrobial activity of the leaf, and rhizome of collected from different altitudes. Phytochemicals such as phenols, flavonoids, flavanol, tannins, emodin, and paeoniflorin were found in all the sampled populations, but the quantity varied significantly across the altitude. In leaf, phenolics, flavonoids, and tannins content positively correlated with altitude ( < 0.01), but flavanol did not show any connection. Similarly, in the rhizome, positive relation with altitude ( < 0.01) was observed in phenol, flavonoids, and paeoniflorin. Antioxidant activity measured by 1, 1-diphenyl- 2 picrylhydrazyl (DPPH) and nitric oxide assays showed a positive correlation ( < 0.05) with altitude. 2, 2-azinobis (3-ethylbenzothiazoline-6-sulphonic acid), ferric reducing antioxidant power, and hydroxyl ion assays did not show any relation with altitude. Antimicrobial activity was higher in the case of rhizome for the minimum inhibitory concentration and positively correlated with phenolics, flavonoids, and flavanol ( < 0.05). The present study further revealed that the secondary metabolites in the leaf and rhizome extracts of are an excellent source of antioxidant and antimicrobial activity, thus validating the species' therapeutic potential.
The online version contains supplementary material available at 10.1007/s12298-022-01242-z.
是喜马拉雅地区具有重要民族治疗价值的植物之一，可用于治疗多种疾病。然而，迄今为止，尚未有关于海拔对其植物化学成分、抗氧化和抗菌活性影响的系统研究报道。本研究评估了从不同海拔采集的该植物叶片和根茎中生物活性化合物、抗氧化和抗菌活性的变化。在所有采样群体中均发现了酚类、黄酮类、黄烷醇、单宁、大黄素和芍药苷等植物化学成分，但含量在不同海拔间存在显著差异。在叶片中，酚类、黄酮类和单宁含量与海拔呈正相关（<0.01），但黄烷醇未显示出任何关联。同样，在根茎中，酚类、黄酮类和芍药苷与海拔呈正相关（<0.01）。通过1,1-二苯基-2-苦基肼（DPPH）和一氧化氮测定法测得的抗氧化活性与海拔呈正相关（<0.05）。2,2-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）、铁还原抗氧化能力和羟自由基测定法与海拔无任何关联。根茎的抗菌活性在最低抑菌浓度方面更高，且与酚类、黄酮类和黄烷醇呈正相关（<0.05）。本研究进一步表明，该植物叶片和根茎提取物中的次生代谢产物是抗氧化和抗菌活性的优质来源，从而验证了该物种的治疗潜力。
在线版本包含可在10.1007/s12298-022-01242-z获取的补充材料。
