Du Jie, Zhang Cuiyang, Long Qingshan, Zhang Liang, Chen Wu, Liu Qingshu
Hunan Provincial Engineering and Technology Research Center for Agricultural Microbiology Application, Hunan Institute of Microbiology, Changsha, China.
College of Plant Protection, Hunan Agricultural University, Changsha, China.
Front Plant Sci. 2022 Oct 25;13:1022476. doi: 10.3389/fpls.2022.1022476. eCollection 2022.
Edeines are a group of non-ribosomal antibacterial peptides produced by . Due to the significant antibacterial properties of edeines, increasing edeine yield is of great interest in biomedical research. Herein, we identified that EdeB, a member of the ParB protein family, significantly improved edeine production in . First, overexpression of in X23 increased edeine production by 92.27%. Second, bacteriostasis experiment showed that -deletion mutant exhibited less antibacterial activity. Third, RT-qPCR assay demonstrated that the expression of , , and , which are key components of the edeine biosynthesis pathway, in -deletion mutant X23(ΔedeB) was significantly lower than that in wild-type strain X23. Finally, electrophoretic mobility shift assay (EMSA) showed that EdeB directly bound to the promoter region of the edeine biosynthetic gene cluster ( BGC), suggesting that EdeB improves edeine production through interaction with BGC in .
伊短菌素是由……产生的一组非核糖体抗菌肽。由于伊短菌素具有显著的抗菌特性，提高伊短菌素产量在生物医学研究中备受关注。在此，我们发现ParB蛋白家族成员EdeB显著提高了……中伊短菌素的产量。首先，在X23中过表达……使伊短菌素产量提高了92.27%。其次，……抑菌实验表明，……缺失突变体表现出较低的抗菌活性。第三，RT-qPCR分析表明，伊短菌素生物合成途径的关键组分……、……和……在……缺失突变体X23(ΔedeB)中的表达显著低于野生型……菌株X23。最后，电泳迁移率变动分析（EMSA）表明，EdeB直接与伊短菌素生物合成基因簇（……BGC）的启动子区域结合，这表明EdeB通过与……中的……BGC相互作用提高了伊短菌素的产量。
