Giaever I
J Immunol Methods. 1978;24(1-2):57-61. doi: 10.1016/0022-1759(78)90086-8.
A new general immunology test is described which is capable of detecting protein in the 1 microgram/ml range. First an antigen is adsorbed in a small area of a glass surface. The glass is exposed to an antibody solution and the antibody will attach specifically to the antigen. Next the slide is washed, dried and sprayed lightly with plastic particles that stick to the protein layers on the glass. Now when the glass slide is exposed to an acid the antigen--antibody bonds are broken, the antibodies will leave the surface removing some of the plastic particles and leaving an easily visible spot behind.
描述了一种新的通用免疫学检测方法，该方法能够检测浓度在1微克/毫升范围内的蛋白质。首先，将抗原吸附在玻璃表面的一小块区域。将玻璃暴露于抗体溶液中，抗体将特异性地附着于抗原。接下来，冲洗玻片，干燥，然后轻轻喷洒塑料颗粒，这些颗粒会粘附在玻璃上的蛋白质层上。现在，当将载玻片暴露于酸中时，抗原-抗体键断裂，抗体将离开表面，带走一些塑料颗粒，留下一个易于看见的斑点。
