Rothen A
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2462-4. doi: 10.1073/pnas.72.6.2462.
Nickel-plated slides were prepared by evaporating a nickel layer (congruent to 4000 A thick) on glass slides in the presence of a magnetic field whose lines of force were perpendicular to the surface of the slides. Such slides are called active. After being coated with a layer of bovine albumin, they could absorb a layer of antibodies 70-80 A thick. However, if the active slides before they were coated with bovine serum albumin, were submitted to a magnetic field with lines of force parallel to the surface, the layer of antibodies absorbed was only 40 A thick. They had become inactive. It has been found that slides remain active at night but that shortly after sunrise they become slowly inactivated and reach a minimum in their activity at exactly the midday period. They regain full activity at sunset. It is shown that the inactivation results from a solar radiation that can be stopped by 3.5 cm of lead. On December 13th, 1974 there was an eclipse of the sun with 65% occultation at noon (Daylight Saving Time). The activity of the slide at noon was 65% of the maximum activity (83 A) observed before sunrise. The thickness of the adsorbed layer of antibodies were 75 A instead of 63 A observed in the absence of the eclipse. The activation of the slides originates in a radiation of non-solar origin that is adsorbed by 1 can of lead.
通过在存在磁力线垂直于载玻片表面的磁场的情况下，在载玻片上蒸发一层厚度相当于4000埃的镍层来制备镀镍载玻片。这样的载玻片被称为活性载玻片。在涂上一层牛血清白蛋白后，它们能够吸附一层厚度为70 - 80埃的抗体。然而，如果在涂上牛血清白蛋白之前，将活性载玻片置于磁力线平行于表面的磁场中，所吸附的抗体层只有40埃厚。它们就变成了非活性的。已经发现载玻片在夜间保持活性，但在日出后不久它们会慢慢失去活性，并在正午时分达到活性的最小值。它们在日落时恢复完全活性。结果表明，失活是由一种能被3.5厘米厚的铅阻挡的太阳辐射引起的。1974年12月13日中午（夏令时）发生了日偏食，掩食率为65%。中午时载玻片的活性是日出前观察到的最大活性（83埃）的65%。所吸附的抗体层厚度为75埃，而在没有日食的情况下观察到的厚度为63埃。载玻片的活化源自一种非太阳起源的辐射，这种辐射能被1罐铅吸收。
