Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, Shizishan Street, Hongshan District, Wuhan, 430070, Hubei, China.
Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, Shizishan Street, Hongshan District, Wuhan, 430070, Hubei, China.
Anal Chim Acta. 2022 Dec 15;1236:340564. doi: 10.1016/j.aca.2022.340564. Epub 2022 Oct 31.
Bacterial detection in foods have to overcome the hindrance of complex matrices, resulting in more sophisticated pre-treatment steps and low sensitivity and reproductivity. Therefore, it remains a challenge to develop simple, rapid, accurate, and low-cost pathogen detection methods which were applicable for detection in turbid or complex food matrices. In this study, a novel bioorthogonal reaction-amplified microparticle counting sensing based on phages has been developed for the rapid detection of viable Salmonella in different foods. Phage not only acted as a bioreceptor to recognize viable Salmonella, but also used as an effective carrier for signal amplifiers due to its large specific surface area. The bioorthogonal reaction was introduced to further amplify the signal, the presence of Salmonella can specifically induce a quantitative change in the functionalized polystyrene microspheres, and thus can be monitored by the microporous resistance particle counter. Under the optimized conditions, this sensor could achieve a limit of detection of 33.58 CFU/mL and a linear range from 10 CFU/mL to 10 CFU/mL. This phage-mediated microporous resistance particle sensor can specifically recognize viable Salmonella in 1 h, providing a powerful platform for rapid and low-cost Salmonella quantification.
在食品中进行细菌检测时，必须克服复杂基质的阻碍，这导致需要更复杂的预处理步骤，并且检测的灵敏度和重现性较低。因此，开发简单、快速、准确且低成本的病原体检测方法仍然是一项挑战，这些方法适用于混浊或复杂的食品基质中的检测。在本研究中，开发了一种新型的基于噬菌体的生物正交反应放大微颗粒计数传感技术，用于快速检测不同食品中的活菌沙门氏菌。噬菌体不仅可以作为生物受体来识别活菌沙门氏菌，而且由于其较大的比表面积，还可以用作信号放大器的有效载体。引入生物正交反应来进一步放大信号，沙门氏菌的存在可以特异性地诱导功能化聚苯乙烯微球发生定量变化，因此可以通过微孔电阻颗粒计数器进行监测。在优化条件下，该传感器的检测限可达 33.58 CFU/mL，线性范围为 10 CFU/mL 至 10 CFU/mL。这种噬菌体介导的微孔电阻颗粒传感器可以在 1 小时内特异性识别活菌沙门氏菌，为快速、低成本的沙门氏菌定量提供了强大的平台。
