Zhang Haotian, Wang Xiaohong, Xu Zuhui, Ma Jimei, Li Zi-Long, Cheng Wan-Min, Jiang Hong
College of Science, Huazhong Agricultural University, Wuhan, 430070, China.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
Anal Bioanal Chem. 2023 May;415(12):2163-2172. doi: 10.1007/s00216-023-04623-9. Epub 2023 Mar 4.
Salmonella enteritidis is one of the most common foodborne pathogens. Many methods have been developed to detect Salmonella, but most of them are expensive, time-consuming, and complex in experimental procedures. Developing a rapid, specific, cost-effective, and sensitive detection method is still demanded. In this work, a practical detection method is presented using salicylaldazine caprylate as the fluorescent probe, which could be hydrolyzed by caprylate esterase liberated from Salmonella lysed by phage, to form strong fluorescent salicylaldazine. The Salmonella could be detected accurately with a low limit of detection of 6 CFU/mL and a broad concentration range of 10-10 CFU/mL. Moreover, this method was successfully used for the rapid detection of Salmonella in milk within 2 h through pre-enrichment by ampicillin-conjugated magnetic beads. The novel combination of fluorescent turn-on probe salicylaldazine caprylate and phage ensures this method has excellent sensitivity and selectivity.
肠炎沙门氏菌是最常见的食源性病原体之一。已经开发了许多检测沙门氏菌的方法，但其中大多数方法昂贵、耗时且实验过程复杂。仍然需要开发一种快速、特异、经济高效且灵敏的检测方法。在这项工作中，提出了一种实用的检测方法，该方法使用辛酸水杨醛腙作为荧光探针，它可以被噬菌体裂解沙门氏菌释放的辛酸酯酶水解，形成强荧光的水杨醛腙。沙门氏菌能够被准确检测，检测限低至6 CFU/mL，浓度范围宽达10⁻¹⁰ CFU/mL。此外，该方法通过氨苄青霉素偶联磁珠进行预富集，成功地在2小时内快速检测了牛奶中的沙门氏菌。荧光开启探针辛酸水杨醛腙与噬菌体的新颖组合确保了该方法具有出色的灵敏度和选择性。
