Physics Department, Emory University, 400 Dowman Dr., Atlanta, Georgia 30322, USA.
Phys Rev E. 2022 Oct;106(4-1):044406. doi: 10.1103/PhysRevE.106.044406.
Transcriptional pausing is highly regulated by the template DNA and nascent transcript sequences. Here, we propose a thermodynamic model of transcriptional pausing, based on the thermal energy of transcription bubbles and nascent RNA structures, to describe the kinetics of the reaction pathways between active translocation, elemental, backtracked, and hairpin-stabilized pauses. The model readily predicts experimentally detected pauses in high-resolution optical-tweezer measurements of transcription. Unlike other models, it also predicts the effect of tension and the GreA transcription factor on pausing.
转录暂停受到模板 DNA 和新生转录本序列的高度调控。在这里，我们提出了一个转录暂停的热力学模型，该模型基于转录泡和新生 RNA 结构的热能，来描述活性转录、基本、回溯和发夹稳定暂停之间反应途径的动力学。该模型可以很容易地预测在高分辨率光镊转录测量中检测到的暂停。与其他模型不同，它还预测了张力和 GreA 转录因子对暂停的影响。
