Wagner M, Wagner B
J Gen Microbiol. 1978 Oct;108(2):283-94. doi: 10.1099/00221287-108-2-283.
The morphological appearance of deproteinized Group A and C streptococcal walls after treatment by different procedures extracting teichoic acids and polysaccharides (formamide, hydrochloric acid, nitrous acid, trichloroacetic acid, sulphuric acid, sodium hydroxide and sodium deoxycholate) was compared with the content of teichoic acids and polysaccharides remaining in the treated walls. All procedures extracted teichoic acids almost completely, but polysaccharides were extracted to various degrees. The ultrastructural appearance of walls after these extractions still exhibited the triple-layered wall profile; only a reduction of thickness of the wall and of electron density of the layers occurred. There was no direct correlation between the reduction of rhamnose content and thickness of walls. The ultrastructural localization of peptidoglycan in the streptococcal walls was explored by means of the indirect immunoferritin technique using anti-peptidoglycan antibodies isolated from anti-Group A-variant antisera. Ferritin particles were bound predominantly to filamentous structures which protruded from both surfaces of peptidoglycan fragments and isolated walls. Peptidoglycan was also detected on the filamentous protrusions of whole cocci. These results contradict models of the streptococcal wall in which peptidoglycan forms the innermost layer and support a mosaic structure in which peptidoglycan forms a network of the peptidoglycan-polysaccharide complex.
将经不同方法（甲酰胺、盐酸、亚硝酸、三氯乙酸、硫酸、氢氧化钠和脱氧胆酸钠）提取磷壁酸和多糖处理后的A组和C组脱蛋白链球菌壁的形态外观，与处理后壁中残留的磷壁酸和多糖含量进行了比较。所有方法几乎都能完全提取磷壁酸，但多糖的提取程度各不相同。这些提取后壁的超微结构外观仍呈现出三层壁的轮廓；只是壁的厚度和各层的电子密度有所降低。鼠李糖含量的减少与壁的厚度之间没有直接相关性。利用从抗A组变异体抗血清中分离出的抗肽聚糖抗体，通过间接免疫铁蛋白技术探索了肽聚糖在链球菌壁中的超微结构定位。铁蛋白颗粒主要结合在从肽聚糖片段和分离壁的两个表面突出的丝状结构上。在整个球菌的丝状突起上也检测到了肽聚糖。这些结果与肽聚糖形成最内层的链球菌壁模型相矛盾，并支持一种肽聚糖形成肽聚糖 - 多糖复合物网络的镶嵌结构模型。
