Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Department of Surgery and Endoscopy, Oral Medicine Research Center, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka, 814-0193, Japan.
Surg Today. 2023 Jun;53(6):663-674. doi: 10.1007/s00595-022-02607-3. Epub 2022 Nov 19.
Analyzing the gut microbiome is essential for planning treatment strategies to manage esophageal squamous cell carcinoma. This study aimed to characterize the gut microbiome of patients with esophageal squamous cell carcinoma and to identify alterations in its composition during treatment.
We observed alterations in the gut microbiome in 21 consecutive patients with esophageal squamous cell carcinoma at five different time points, from neoadjuvant treatment to postoperative surgery. Ten healthy individuals were used as a non-cancer control group. Fecal samples were collected and analyzed using 16S ribosomal ribonucleic acid sequencing.
Before treatment, participants with esophageal squamous cell carcinoma had different alpha and beta diversity in comparison to healthy controls. The number of Streptococcus, a facultative anaerobic bacterium, was significantly higher, whereas that of Faecalibacterium, an obligate anaerobic bacterium, was significantly lower. Both alpha and beta diversity remained unchanged during neoadjuvant treatment, but the alterations were pronounced after surgery. The increase in the relative abundance of Streptococcus and the decrease in that of Faecalibacterium also tended to be more pronounced after surgery.
The gut microbiome in patients with esophageal squamous cell carcinoma is altered with surgical intervention.
分析肠道微生物组对于制定治疗策略以管理食管鳞状细胞癌至关重要。本研究旨在描述食管鳞状细胞癌患者的肠道微生物组，并确定其在治疗过程中的组成变化。
我们观察了 21 例连续的食管鳞状细胞癌患者在五个不同时间点（从新辅助治疗到术后手术）的肠道微生物组变化。10 名健康个体作为非癌症对照组。采集粪便样本并使用 16S 核糖体 RNA 测序进行分析。
在治疗前，与健康对照组相比，食管鳞状细胞癌患者的α和β多样性存在差异。兼性厌氧菌链球菌的数量明显更高，而专性厌氧菌粪杆菌的数量明显更低。在新辅助治疗期间，α和β多样性保持不变，但手术后变化明显。链球菌相对丰度的增加和粪杆菌相对丰度的减少也倾向于在手术后更为明显。
手术干预会改变食管鳞状细胞癌患者的肠道微生物组。
